Abstract Nowadays, an increasing amount of studies indicate the numerous applications of bacteriophages (e.g. medical, pharmaceutical, use in animal breeding, food processing). However, so far, there is limited availability of simple, efficient, and sensitive methods for evaluation and comparison of the phage’s lytic activity against its host. The aim of this paper is the examination of a new methodological approach (PhageScore method) for the comparative evaluation of bacteriophages lytic activity against bacterial host. For this purpose, two model bacteria (gram-positive Staphylococcus aureus and gram-negative Escherichia coli) were cultivated under control conditions and with the presence of proper bacteriophages (three kayviruses for S. aureus and three wildtypes of T4-like phages infecting E. coli) with the different initial multiplicity of infection (MOI, ranging from MOI = 0.1–1 for S. aureus, and MOI = 0.01–1 for E. coli). The experimental data were analyzed using the proposed method, which employed a set of appropriate mathematical expressions. The results obtained by the proposed procedure clearly show differences between phage lytic activity. Thus, the PhageScore method provides a useful tool for bacteriophage characterization, giving a precise rate of lytic activity which can be useful for a comparison between bacteriophages for selected purposes.

中文翻译：

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PhageScore：一种比较评估噬菌体裂解活性的简单方法

摘要 如今，越来越多的研究表明噬菌体的众多应用（例如医学、制药、动物育种、食品加工）。然而，到目前为止，用于评估和比较噬菌体对其宿主的裂解活性的简单、有效和灵敏的方法的可用性有限。本文的目的是检查一种新的方法学方法（PhageScore 方法），用于比较评估噬菌体对细菌宿主的裂解活性。为此，在对照条件下培养两种模型细菌（革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌）并存在适当的噬菌体（三种用于金黄色葡萄球菌的 kayviruses 和感染大肠杆菌的三种 T4 样噬菌体的野生型。大肠杆菌）具有不同的初始感染复数（MOI，金黄色葡萄球菌的 MOI = 0.1-1，大肠杆菌的 MOI = 0.01-1）。使用所提出的方法分析了实验数据，该方法采用了一组适当的数学表达式。通过所提出的程序获得的结果清楚地显示了噬菌体裂解活性之间的差异。因此，PhageScore 方法为噬菌体表征提供了一种有用的工具，提供了精确的裂解活性速率，可用于比较噬菌体之间的选择目的。通过所提出的程序获得的结果清楚地显示了噬菌体裂解活性之间的差异。因此，PhageScore 方法为噬菌体表征提供了一种有用的工具，提供了精确的裂解活性速率，可用于比较噬菌体之间的选择目的。通过所提出的程序获得的结果清楚地显示了噬菌体裂解活性之间的差异。因此，PhageScore 方法为噬菌体表征提供了一种有用的工具，提供了精确的裂解活性速率，可用于比较噬菌体之间的选择目的。