This study is a first outcome of a series of works on immobilized cultures of Chlorella vulgaris. Colonies were grown independently on solid media under heterotrophic conditions and their growth was followed by continuous three-dimensional microscopy and image analysis. The growth was assessed by 3D imaging using a structured light microscope and subsequent image processing. Based on the expressions proposed for the height and radius growth dynamics, we concluded that the colonies expanded at a constant rate in the horizontal direction and a decreasing rate in the vertical direction. A spherical cap best describes the shape of the colonies during the growth period. During development, the packing density of the cells in the colony was calculated to occupy 25–40% of the available space. The cell yield was initially much lower than published values and approached those values at the end, indicating that the glucose consumption was dependent on not only the growth and division of cells but also on the activity of old cells. Finally, no colony-colony intraspecies interaction was observed when two colonies were grown in proximity.

这项研究是关于小球藻固定化培养物的一系列研究的第一个成果。菌落在异养条件下在固体培养基上独立生长，并通过连续的三维显微镜和图像分析追踪其生长。通过使用结构化光学显微镜的3D成像和随后的图像处理来评估生长。基于提出的关于高度和半径生长动力学的表达式，我们得出结论，菌落在水平方向上以恒定速率扩展，而在垂直方向上以递减速率扩展。球形帽最能描述生长期的菌落形状。在发育过程中，计算出菌落中细胞的堆积密度占可用空间的25-40％。最初的单元格产量远低于公布的值，最后达到了这些值，表明葡萄糖的消耗不仅取决于细胞的生长和分裂，还取决于旧细胞的活性。最后，当两个菌落邻近生长时，没有观察到菌落-菌落种间相互作用。