Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is the natural vector of the Huanglongbing (HLB) pathogen that presents an unparalleled challenge to citrus production. RNA interference (RNAi) has already shown great potential for insect pest management owing to its high specificity. RNAi technique relies on the delivery of exogenous double-stranded RNAs (dsRNAs) that target essential genes in insects. The D. citri genome possesses three dsRNase homologs and all core RNAi machinery genes, aside from the R2D2 that functions as a cofactor of Dicer-2 in the siRNA pathway. Nevertheless, ever-increasing research on the application of RNAi to suppress D. citri population has promulgated outstanding progress. Here, we provide a comprehensive review of the recent literature, and summarize the current understanding of RNAi mechanisms and advances in the applications of RNAi in D. citri . The major challenges including the potential of dsRNA degradation, target gene selection, and dsRNA delivery methods, as well as the implications of using RNAi for D. citri control are reviewed.

中文翻译：

---

RNA干扰介导控制亚洲柑橘木虱，黄龙病细菌病原体的载体

亚洲柑橘木虱，Diaphorina citri Kuwayama（半翅目：Liviidae）是黄龙病（HLB）病原体的天然载体，对柑橘生产提出了无与伦比的挑战。RNA 干扰 (RNAi) 由于其高度特异性，已经在害虫管理中显示出巨大的潜力。RNAi 技术依赖于外源性双链 RNA (dsRNA) 的传递，这些 RNA 靶向昆虫中的必需基因。D. citri 基因组拥有三个 dsRNase 同源物和所有核心 RNAi 机器基因，除了在 siRNA 途径中作为 Dicer-2 辅助因子的 R2D2。然而，越来越多的关于应用 RNAi 抑制 D. citri 种群的研究已经取得了显着进展。在这里，我们全面回顾了最近的文献，并总结目前对 RNAi 机制的理解以及 RNAi 在柑橘属植物中的应用进展。主要挑战包括 dsRNA 降解的潜力、目标基因选择和 dsRNA 递送方法，以及使用 RNAi 控制柑橘类果蝇的影响。