Diet-induced obesity (DIO) has been shown to increase DNA methyltransferases (DNMTs) expression and DNMTs binding at obesity-associated genes. Natural compounds have the potential to reverse obesity-associated gene expression via the regulation of DNA methylation. The objective of this study was to determine the effect of health promoting compounds of flaxseed on DNMTs and obesity-associated gene expression and weight gain. Sixty C57BL/6J male mice were randomly assigned into one of the following diet groups and fed for eight weeks: 45% kcal fat; 45% kcal fat+10% whole flaxseed; 45% kcal fat+6% defatted flaxseed; 45% kcal fat+4% flaxseed oil; and 16% kcal fat. DNMT1, DNMT3a, DNMT3b, leptin, and peroxisome proliferator-activated receptor (PPAR)-α expressions in adipose and muscle tissues were determined by real-time PCR. The health promoting compounds of flaxseed affected selected gene expression and attenuated weight gain. Further research is needed to identify the specific mechanisms modulating leptin or PPAR-α expression during DIO development.

中文翻译：

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亚麻籽的健康促进化合物是否通过调节肥胖基因表达来减轻体重增加？

饮食诱导的肥胖 (DIO) 已被证明会增加 DNA 甲基转移酶 (DNMT) 的表达和 DNMT 在肥胖相关基因上的结合。天然化合物有可能通过调节 DNA 甲基化来逆转肥胖相关基因的表达。本研究的目的是确定亚麻籽促进健康的化合物对 DNMT 和肥胖相关基因表达和体重增加的影响。60 只 C57BL/6J 雄性小鼠被随机分配到以下饮食组之一并喂食 8 周： 45% kcal 脂肪；45% 大卡脂肪 + 10% 全亚麻籽；45%大卡脂肪+6%脱脂亚麻籽；45%大卡脂肪+4%亚麻籽油；和 16% 千卡脂肪。通过实时 PCR 测定脂肪和肌肉组织中 DNMT1、DNMT3a、DNMT3b、瘦素和过氧化物酶体增殖物激活受体 (PPAR)-α 的表达。亚麻籽的健康促进化合物影响选定的基因表达并减弱体重增加。需要进一步研究以确定在 DIO 发育过程中调节瘦素或 PPAR-α 表达的具体机制。