Silver nanoparticles (AgNPs) are widely used in many applications of biotechnology, including medicine and agriculture. They are released to the nature as waste materials, which can cause physiological and biochemical effects on plants. Indigo, indirubin and tryptanthrin are valuable indole alkaloid compounds in Isatis constricta due to both medicinal effects and dye properties. This research was conducted to determine the effects of different concentrations (0, 0.25, 0.5, 1, 1.5 and 2 mg L⁻¹) of AgNPs on the production of indigo, indirubin and tryptanthrin compounds in leaves of in vitro grown shoots of I. constricta Davis. Indigo production was 1.15-fold of control (869 ± 8.33 µg g⁻¹) in the leaves of shoots regenerated in Murashige and Skoog supplemented with 2 mg L⁻¹ of AgNPs (1003 ± 11.42 µg g⁻¹) on 5 days post-treatment. Tryptanthrin production showed an increase in all applications of AgNPs, but the highest increase was observed at a concentration of 2 mg L⁻¹ (4.59 ± 0.046 µg g⁻¹) and this increase was 1.71-fold of control (2.68 ± 0.031 µg g⁻¹) on 5 days post-treatment. The production of indigo and tryptanthrin decreased on 10 and 15 days post-treatment with AgNPs. The contents of indirubin decreased during day 5-10-15 and at all concentrations of AgNPs compared to the control.

中文翻译：

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银纳米颗粒对板蓝根中吲哚生物碱产生的影响

银纳米颗粒（AgNPs）广泛用于生物技术的许多应用，包括医学和农业。它们作为废物释放到自然中，可能对植物造成生理和生化作用。靛蓝，靛玉红和色胺酮是有价值吲哚生物碱化合物菘缢由于两个药效和染料的性质。进行了这项研究以确定不同浓度（0、0.25、0.5、1、1.5和2 mg L ^-1）的AgNPs对I体外生长芽中靛蓝，靛玉红和色胺酮化合物产生的影响。收缩戴维斯。靛蓝产量是对照的1.15倍（869±8.33 µg g ^-1）处理后5天，在补充2 mg L ^-1的AgNPs（1003±11.42 µg g ^-1）的Murashige和Skoog中再生的枝条叶片中。Tryptanthrin的生产显示出所有应用的AgNP均增加，但在2 mg L ^-1（4.59±0.046 µg g ^-1）的浓度下观察到最大的增加，并且该增加是对照（2.68±0.031 µg g）的1.71倍^-1）在治疗后5天。用AgNP处理后第10天和第15天，靛蓝和色胺酮的产量下降。与对照相比，在5-10-15天以及所有AgNPs浓度下，靛玉红含量均降低。