Methanol production by co-culture of methanotrophs Methylocystis bryophila and Methyloferula stellata was examined from methane, a greenhouse gas. Co-culture exhibited higher methanol yield of 4.72 mM at optimum ratio of M. bryophila and M. stellata (3:2) compared to individual cultures. The immobilized co-culture within polyvinyl alcohol (PVA) showed relative efficiency of 90.1% for methanol production at polymer concentration of 10% (v/v). The immobilized co-culture cells within PVA resulted in higher bioprocess stability over free cells at different pH, and temperatures. Free and encapsulated co-cultures showed maximum methanol production of 4.81 and 5.37 mM under optimum conditions, respectively. After five cycles of reusage under batch conditions, free and encapsulated co-cultures retained methanol production efficiency of 23.8 and 61.9%, respectively. The present investigation successfully revealed the useful influence of co-culture on the methanol production over pure culture. Further, encapsulation within the polymeric matrix proved to be a better approach for the enhanced stability of the bioprocess.

中文翻译：

---

通过甲烷氧化菌的固定化共培养物从甲烷生产生物甲醇。

甲烷氧化菌Methylocystis bryophila和Methyloferula stellata共培养的甲醇生产是从甲烷（一种温室气体）中检测出来的。在M. bryophila和M. stellata 的最佳比例下，共培养表现出更高的甲醇产量，为 4.72 mM(3:2) 与个体文化相比。聚乙烯醇 (PVA) 内的固定化共培养物在聚合物浓度为 10% (v/v) 时显示出 90.1% 的甲醇生产相对效率。在不同的 pH 值和温度下，PVA 内的固定化共培养细胞比游离细胞具有更高的生物过程稳定性。在最佳条件下，游离和封装的共培养物的最大甲醇产量分别为 4.81 和 5.37 mM。在分批条件下重复使用五个循环后，游离和封装的共培养物分别保留了 23.8% 和 61.9% 的甲醇生产效率。目前的调查成功地揭示了共培养对甲醇生产的有益影响，而不是纯培养。更远，