Today, industrial activities lead to the accumulation of heavy metals in the soil, water, and air due to mine deposits and operations, fertilizers, and drugs used in agriculture, and urban wastes. Using microorganism bioremediation of metals is an important technique in solving these problems. Herein, a rhizoid bacterium isolated from orchids that grow in Ovit plateau was defined as Bacillus sp. 5O5Y11 by conventional and molecular methods and the bioremediation properties of strain were investigated. It was capable of growth at high salt (10–15%) concentration, wide temperature (10–45 °C) and pH range (pH 4.5–8.0), and was observed to have strong lecithinase, gelatinase activity, and nitrate reduction. When the plant growth-promoting properties of this strain were examined, strong siderophore and ammonium production were observed in in vitro conditions. Bacillus sp. 5O5Y11 was found to have high tolerance to a group of heavy metals [iron (Fe), copper (Cu), lead (Pb), silver (Ag), zinc (Zn)]. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) values of copper metal on Bacillus sp. 5O5Y11 were determined as 12.5 mM and 50 mM, respectively. The effectiveness of this bacterium on the germination and growth of maize plant in the presence and absence of copper were investigated. These results suggest that Bacillus sp. 5O5Y11 is a microorganism, which has potential in metal bioremediation and plant growth promotion.

中文翻译：

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土传芽孢杆菌生物修复特性的测定。铜存在下5O5Y11及其对玉米发育的影响

今天，工业活动导致重金属在土壤、水和空气中积累，原因是矿藏和作业、化肥、农业中使用的药物以及城市废物。利用微生物对金属进行生物修复是解决这些问题的重要技术。在本文中，从生长在奥维特高原的兰花中分离出的一种根状细菌被定义为芽孢杆菌。通过常规方法和分子方法研究了5O5Y11和菌株的生物修复特性。它能够在高盐 (10-15%) 浓度、宽温度 (10-45 °C) 和 pH 值范围 (pH 4.5-8.0) 下生长，并观察到具有很强的卵磷脂酶、明胶酶活性和硝酸盐还原能力。当检查该菌株的植物生长促进特性时，在体外条件下观察到强铁载体和铵的产生。芽孢杆菌 发现 5O5Y11 对一组重金属 [铁 (Fe)、铜 (Cu)、铅 (Pb)、银 (Ag)、锌 (Zn)] 具有高耐受性。铜金属对芽孢杆菌的最小抑制浓度 (MIC) 和最小杀菌浓度 (MBC) 值。5O5Y11 分别测定为 12.5 mM 和 50 mM。研究了这种细菌在存​​在和不存在铜的情况下对玉米植物的萌发和生长的有效性。这些结果表明芽孢杆菌属。5O5Y11是一种微生物，在金属生物修复和促进植物生长方面具有潜力。铜金属对芽孢杆菌的最小抑制浓度 (MIC) 和最小杀菌浓度 (MBC) 值。5O5Y11 分别测定为 12.5 mM 和 50 mM。研究了这种细菌在存​​在和不存在铜的情况下对玉米植物的萌发和生长的有效性。这些结果表明芽孢杆菌属。5O5Y11是一种微生物，在金属生物修复和促进植物生长方面具有潜力。铜金属对芽孢杆菌的最小抑制浓度 (MIC) 和最小杀菌浓度 (MBC) 值。5O5Y11 分别测定为 12.5 mM 和 50 mM。研究了这种细菌在存​​在和不存在铜的情况下对玉米植物的萌发和生长的有效性。这些结果表明芽孢杆菌属。5O5Y11是一种微生物，在金属生物修复和促进植物生长方面具有潜力。