The aim of this study was to assess the vaccine-matching and antigenic properties of foot-and-mouth disease virus (FMDV) isolates collected from Ethiopia between 2011 and 2014. Samples (n = 51) were collected from cattle and pigs with clinical signs consistent with foot-and-mouth disease (FMD) on farms in Debre-Berhan, Debre-Zeit/Bishoftu, Sidamo, Mekelle, and Addis Ababa. Infectious FMDV was isolated using BHK-21 cell cultures from 38 of the 51 field samples (74.5%). All of these FMDV-positive samples were characterized as serotype O, belonging to two East Africa topotypes (EA-3 and EA-4), and their VP1-encoding sequences demonstrated amino acid sequence variability encompassing 27 positions in comparison to the vaccine strain (O/ETH/38/2005) currently provided by the National Veterinary Institute of Ethiopia. One-dimensional virus neutralization test (1 dm VNT) results showed that O/ETH/38/2005 was antigenically matched to 10 of the 16 serotype O viruses. These findings indicate that the O/ETH/38/2005 vaccine strain can provide protection against outbreaks caused by the O/EA-3 topotype, although poorer vaccine-matching results for the O/EA-4 topotype reinforce the importance of using a good-quality vaccine with high coverage in the susceptible herds with supporting post-vaccination serosurveillance to ensure that sufficient antibody titers are generated in the vaccinated animals.

中文翻译：

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对 2011 年至 2014 年在埃塞俄比亚分离的 O 血清型口蹄疫病毒不同基因变异的疫苗匹配评估。

本研究的目的是评估 2011 年至 2014 年间从埃塞俄比亚采集的口蹄疫病毒 (FMDV) 分离株的疫苗匹配和抗原特性。样本 (n = 51) 采集自有临床症状的牛和猪与 Debre-Berhan、Debre-Zeit/Bishoftu、Sidamo、Mekelle 和亚的斯亚贝巴农场的口蹄疫 (FMD) 一致。使用 BHK-21 细胞培养物从 51 个现场样本中的 38 个样本中分离出传染性 FMDV（74.5%）。所有这些 FMDV 阳性样本均被定性为 O 血清型，属于两种东非拓扑型（EA-3 和 EA-4），与疫苗株相比，它们的 VP1 编码序列显示出包含 27 个位置的氨基酸序列变异性（ O/ETH/38/2005）目前由埃塞俄比亚国家兽医研究所提供。一维病毒中和试验（1 dm VNT）结果表明，O/ETH/38/2005 与 16 种 O 血清型病毒中的 10 种抗原匹配。这些发现表明，O/ETH/38/2005 疫苗株可以针对 O/EA-3 拓扑型引起的疫情提供保护，尽管 O/EA-4 拓扑型较差的疫苗匹配结果强调了使用良好疫苗株的重要性。 - 在易感群体中具有高覆盖率的优质疫苗，并支持疫苗接种后血清监测，以确保在接种疫苗的动物中产生足够的抗体滴度。