3D bioprinting is an emerging biofabrication process for the production of adherent cell-based products, including engineered tissues and foods. While process innovations are rapidly occurring in the area of process monitoring, which can improve fundamental understanding of process-structure-property relations as well as product quality by closed-loop control techniques, in-line sensing of the bioink composition remains a challenge. Here, we report that hollow multifunctional fibers enable in-line impedimetric sensing of bioink composition and exhibit selectivity for real-time classification of cell type, viability, and state of differentiation during bioprinting. Continuous monitoring of the fiber impedance magnitude and phase angle response from 10 2 to 10 6 Hz during microextrusion 3D bioprinting enabled compositional and quality analysis of alginate bioinks that contained fibroblasts, neurons, or mouse embryonic stem cells (mESCs). Fiber impedimetric resp...

中文翻译：

---

使用空心多功能纤维阻抗传感器进行 3D 生物打印。

3D 生物打印是一种新兴的生物制造工艺，用于生产贴壁细胞产品，包括工程组织和食品。虽然工艺创新在工艺监控领域迅速发生，这可以通过闭环控制技术提高对工艺-结构-性能关系以及产品质量的基本理解，但生物墨水成分的在线传感仍然是一个挑战。在这里，我们报告说，中空多功能纤维能够对生物墨水成分进行在线阻抗传感，并在生物打印过程中对细胞类型、活力和分化状态的实时分类表现出选择性。在微挤压 3D 生物打印过程中连续监测 10 2 至 10 6 Hz 的纤维阻抗幅度和相位角响应，能够对含有成纤维细胞、神经元或小鼠胚胎干细胞 (mESC) 的藻酸盐生物墨水进行成分和质量分析。纤维阻抗测试仪