In this paper, we describe the application of the 4D biofabrication approach for the fabrication of artificial nerve graft. Bilayer scaffolds consisting of uniaxially aligned polycaprolactone-poly(glycerol sebacate) (PCL-PGS) and randomly aligned methacrylated hyaluronic acid (HA-MA) fibers were fabricated using electrospinning and further used for the culture of PC-12 neuron cells. Tubular structures form instantly after immersion of fibrous bilayer in an aqueous buffer and the diameter of obtained tubes can be controlled by changing bilayer parameters such as the thickness of each layer, overall bilayer thickness, and medium counterion concentration. Designed scaffolds showed a self-folded scroll-like structure with high stability after four weeks of real-time degradation. The significance of this research is in the fabrication of tuneable tubular nerve guide conduits that can simplify the current existing clinical treatment of neural injuries.

中文翻译：

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纤维人工神经移植物的4D生物制造，用于神经元再生。

在本文中，我们描述了4D生物制造方法在人工神经移植物制造中的应用。使用电纺丝制造了由单轴排列的聚己内酯-聚癸二酸甘油酯（PCL-PGS）和随机排列的甲基丙烯酸化的透明质酸（HA-MA）纤维组成的双层支架，并将其进一步用于PC-12神经元细胞的培养。将纤维双层浸入水性缓冲液后立即形成管状结构，并且可以通过更改双层参数（例如每层的厚度，双层总厚度和中等抗衡离子浓度）来控制获得的试管的直径。经过四周的实时降解后，设计的支架显示出具有高稳定性的自折叠式涡旋状结构。