The production of different size and shape silver nanoparticles (AgNPs) has increased considerably in recent years due to several commercial and biological applications. Here, rod-shaped AgNPs (SNRs) were prepared using the microwave-assisted method and characterised by ultraviolet-visible spectroscopy, and transmission electron microscopy analysis. The present study aims to investigate the cyto-genotoxic effect of various concentrations (5, 10, and 15 µM) of SNRs using Allium cepa model. As a result, concentration-dependent cyto-genotoxic effect of SNRs was observed through a decrease in the mitotic index, and an increase in the chromosomal aberrations such as chromosome break, disturbed metaphase, and anaphase bridge. To check the impact of Ag+ ions, 15 µM silver nitrate (AgNO3) was prepared and tested in all the assays. Furthermore, cell viability and different reactive oxygen species assays were performed to test the cytotoxicity evaluation of SNRs. The authors found that in all the tested assays, SNRs at high concentrations (15 µM) and AgNO3 (15 µM) were observed to cause maximal damage to the roots. Therefore, the current study implies that the cytotoxicity and genotoxicity of SNRs were dependent on the concentration of SNRs.

中文翻译：

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银纳米棒在洋葱模型中诱导氧化应激和染色体畸变。


近年来，由于多种商业和生物应用，不同尺寸和形状的银纳米颗粒（AgNP）的产量大幅增加。在这里，使用微波辅助方法制备了棒状银纳米粒子（SNR），并通过紫外-可见光谱和透射电子显微镜分析进行了表征。本研究旨在使用洋葱模型研究不同浓度（5、10 和 15 µM）SNR 的细胞基因毒性效应。结果，通过有丝分裂指数的降低和染色体畸变（如染色体断裂、中期扰乱和后期桥）的增加，观察到了 SNR 的浓度依赖性细胞基因毒性效应。为了检查 Ag+ 离子的影响，在所有测定中制备并测试了 15 µM 硝酸银 (AgNO3)。此外，还进行了细胞活力和不同活性氧测定，以测试信噪比的细胞毒性评估。作者发现，在所有测试的测定中，观察到高浓度 (15 µM) 的 SNR 和 AgNO3 (15 µM) 对根部造成最大损害。因此，目前的研究表明，SNR 的细胞毒性和遗传毒性取决于 SNR 的浓度。