The exact mechanics of specialized metabolism and its importance throughout plant evolution remain mysterious. Specialized metabolites and their corresponding biosynthetic genes are crucial to understand the reason for the prevalence of certain metabolism. Even though mass spectrometry-based metabolomics has enabled us to acquire data about the structural properties of unknown specialized metabolites as well as known metabolites and their corresponding isomers/analogs, extensive analytical approaches are still required. Herein, we review the most advanced analytical approaches using stable isotope labeling that can be used to identify the unknown specialized metabolites.

中文翻译：

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使用稳定同位素标记的高维代谢组学，用于鉴定植物中缺失的特殊代谢。

专门代谢的确切机制及其在整个植物进化中的重要性仍然是个谜。专门的代谢物及其相应的生物合成基因对于了解某些代谢盛行的原因至关重要。尽管基于质谱的代谢组学使我们能够获取有关未知专门代谢物以及已知代谢物及其相应异构体/类似物的结构特性的数据，但仍需要广泛的分析方法。在本文中，我们回顾了使用稳定同位素标记的最先进的分析方法，该方法可用于鉴定未知的专门代谢物。