A concept of the comprehensive assessment of soil contamination is proposed. According to it, the conclusion regarding the presence of toxic substances in the analyzed sample is based on the inhibition of enzymatic reactions responsible for various functions of a living organism, such as luminescence, respiration, etc. These functions are taken as test functions in classical bioassays with the use of living objects (luminous bacteria, daphnia, algae, and others). The regularities of the impact of different classes of toxicants on the activity of particular enzymes or coupled oligo-enzyme chains have been established. These enzyme reactions are selected as potential test objects: markers of contamination. Three enzyme systems with the maximal sensitivity to different classes of toxicants have been chosen for the set of enzymatic bioassays: butyrylcholinesterase, NAD(P)H:FMN-oxidoreductase + luciferase, and lactate dehydrogenase + NAD(P)H:FMN-oxidoreductase + luciferase. The possibility to use enzymes instead of living organisms in the bioassay of natural complex systems has been shown.

中文翻译：

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一套用于评估土壤污染的酶促生物测定法。

提出了土壤污染综合评价的概念。据此，有关被分析样品中有毒物质的结论是基于抑制负责生物体各种功能（例如发光，呼吸等）的酶促反应。这些功能被视为经典中的测试功能。使用活体（发光细菌，水蚤，藻类等）的生物测定。已经确定了不同种类的毒物对特定酶或偶联的寡酶链的影响的规律。这些酶反应被选作潜在的测试对象：污染的标志。选择了三种对不同种类的毒物具有最高敏感性的酶系统进行酶促生物测定：丁酰胆碱酯酶，NAD（P）H：FMN氧化还原酶+萤光素酶和乳酸脱氢酶+ NAD（P）H：FMN氧化还原酶+萤光素酶。已经显示了在天然复杂系统的生物测定中使用酶代替活生物体的可能性。