Abstract

Porcine enteric coronaviruses (CoVs) cause highly contagious enteric diarrhea in suckling piglets. These COV infections are characterized by clinical signs of vomiting, watery diarrhea, dehydration, and high morbidity and mortality, resulting in significant economic losses and tremendous threats to the pig farming industry worldwide. Because the clinical manifestations of pigs infected by different CoVs are similar, it is difficult to differentiate between the specific pathogens. Effective high-throughput detection methods are powerful tools used in the prevention and control of diseases. The immune system of piglets is not well developed, so serological methods to detect antibodies against these viruses are not suitable for rapid and early detection. This paper reviews various PCR-based methods used for the rapid and efficient detection of these pathogenic CoVs in swine intestines.

Key points

1. 1.

   Swine enteric coronaviruses (CoVs) emerged and reemerged in past years.

2. 2.

   Enteric CoVs infect pigs at all ages with high mortality rate in suckling pigs.

3. 3.

   Rapid and efficient detection methods are needed and critical for diagnosis.

中文翻译：

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病原性猪肠道冠状病毒的快速高效检测方法。

摘要

猪肠道冠状病毒 (CoVs) 在哺乳仔猪中引起高度传染性肠道腹泻。这些 COV 感染的特点是呕吐、水样腹泻、脱水以及高发病率和死亡率的临床症状，给全球养猪业造成重大经济损失和巨大威胁。由于不同冠状病毒感染的猪的临床表现相似，很难区分具体的病原体。有效的高通量检测方法是预防和控制疾病的有力工具。仔猪的免疫系统不发达，因此检测这些病毒抗体的血清学方法不适合快速和早期检测。

关键点

1. 1.

   猪肠道冠状病毒 (CoVs) 在过去几年中出现并再次出现。

2. 2.

   肠道冠状病毒感染所有年龄的猪，在乳猪中死亡率很高。

3. 3.

   需要快速有效的检测方法，并且对于诊断至关重要。