Disinfection and antisepsis are of primary importance in controlling nosocomial infections and outbreaks by pathogens expressing multiple resistance to antimicrobial agents (multidrug-resistant [MDR]) used in therapy. Nowadays, infections related to health services (HAIs) due to MDR and multidrug-susceptible (MDS) Corynebacterium striatum should not be underestimated, including patients using invasive medical devices. The virulence potential of C. striatum needs further investigation. Currently, susceptibility profiles of planktonic and/or sessile forms of four C. striatum strains of different pulsed-field gel electrophoresis types were examined as biocides based on the manufacturer's recommendations: 2% glutaraldehyde (GA), 2% peracetic acid (PA), 1% potassium monopersulfate (Virkon^®; VK), 1% sodium hypochlorite (SH), and 70% ethyl alcohol (ET). Time-kill assays using 2% bovine serum albumin (BSA) were performed for evaluation of influence of organic matter on biocides effects. Planktonic forms expressed GA resistance at different levels. C. striatum viability was observed until 2, 4, 20, and 30 min for MDR 2369/II, MDS 1954/IV, MDR 1987/I, and MDS 1961/III strains, respectively. In contrast to GA, the biocides PA, VK24h, SH, and ET had higher effective bacterial mortality. However, storage of VK (48 hr) reduced their biocide activities. Moreover, mature biofilms were produced on abiotic substrates, including steel surfaces. Post-treatment with GA (30 min), survival of sessile forms was ≥100% than planktonic forms of all C. striatum tested strains. Independent of biocides tested, BSA increased the survival of planktonic and sessile forms (p ≤ 0.005). Present data indicated that hospital staff should be aware of dissemination and eradication of HAIs by C. striatum presenting resistance to biocides, including high-level disinfectants, such as GA.

中文翻译：

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对浮游和生物膜相关形式的棒状杆菌的防腐剂和消毒剂的抗性。

消毒和防腐对于控制医院内感染和由对治疗中使用的抗菌药物（多重耐药 [MDR]）表现出多重耐药性的病原体引起的爆发至关重要。如今，不应低估由 MDR 和多药敏感 (MDS)棒状杆菌引起的与卫生服务 (HAI) 相关的感染，包括使用侵入性医疗设备的患者。纹状体的毒力潜力需要进一步研究。目前，四种C. 纹状体的浮游和/或无柄形式的敏感性特征根据制造商的建议，将不同脉冲场凝胶电泳类型的菌株作为杀菌剂进行了检查：2% 戊二醛 (GA)、2% 过乙酸 (PA)、1% 单过硫酸钾 (Virkon ^® ; VK)、1% 次氯酸钠（ SH) 和 70% 乙醇 (ET)。使用 2% 牛血清白蛋白 (BSA) 进行时间杀灭试验，以评估有机物对杀生物剂效果的影响。浮游形式表达了不同水平的 GA 抗性。纹状体对于 MDR 2369/II、MDS 1954/IV、MDR 1987/I 和 MDS 1961/III 菌株，分别在 2、4、20 和 30 分钟前观察到活力。与 GA 相比，杀菌剂 PA、VK24h、SH 和 ET 具有更高的有效细菌死亡率。然而，VK 的储存（48 小时）降低了它们的杀生物活性。此外，成熟的生物膜是在非生物基质上产生的，包括钢表面。用 GA 后处理（30 分钟），无柄形式的存活率比所有C. 纹状体测试菌株的浮游形式≥100% 。与测试的杀生物剂无关，BSA 增加了浮游和无柄形式的存活率 ( p  ≤ 0.005)。目前的数据表明，医院工作人员应该意识到纹状体对 HAI 的传播和根除 表现出对杀生物剂的抗性，包括高水平消毒剂，例如 GA。