Heat shock protein (HSP) is a family of highly conserved protein, which exists widely in various organisms and has a variety of important physiological functions. Currently, there is no systematic analysis of HSPs in human glioma. The aim of this study was to investigate the characteristics of HSPs through constructing protein–protein interaction network (PPIN) considering the expression level of HSPs in glioma. After the identification of the differentially expressed HSPs in glioma tissues, a specific PPIN was constructed and found that there were many interactions between the differentially expressed HSPs in glioma. Subcellular localization analysis shows that HSPs and their interacting proteins distribute from the cell membrane to the nucleus in a multilayer structure. By functional enrichment analysis, gene ontology analysis, and Kyoto Encyclopedia of Genes and Genomes pathway analysis, the potential function of HSPs and two meaningful enrichment pathways was revealed. In addition, nine HSPs (DNAJA4, DNAJC6, DNAJC12, HSPA6, HSP90B1, DNAJB1, DNAJB6, DNAJC10, and SERPINH1) are prognostic markers for human brain glioma. These analyses provide a full view of HSPs about their expression, biological process, as well as clinical significance in glioma.

中文翻译：

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胶质瘤中热激蛋白差异表达的网络分析。

热休克蛋白（HSP）是一个高度保守的蛋白家族，广泛存在于各种生物中，具有多种重要的生理功能。目前，尚无对人脑胶质瘤中HSP的系统分析。这项研究的目的是通过考虑神经胶质瘤中HSPs的表达水平，通过构建蛋白-蛋白相互作用网络（PPIN）来研究HSPs的特征。在鉴定了神经胶质瘤组织中差异表达的HSP后，构建了特异性的PPIN，发现在神经胶质瘤中差异表达的HSP之间存在许多相互作用。亚细胞定位分析表明，HSP及其相互作用的蛋白质以多层结构从细胞膜分布到细胞核。通过功能富集分析，基因本体分析，以及《京都基因与基因组百科全书》途径分析，揭示了HSPs的潜在功能和两个有意义的富集途径。此外，九种HSP（DNAJA4，DNAJC6，DNAJC12，HSPA6，HSP90B1，DNAJB1，DNAJB6，DNAJC10和SERPINH1）是人脑神经胶质瘤的预后标志物。这些分析提供了有关HSPs在神经胶质瘤中的表达，生物学过程以及临床意义的完整信息。