Fluorescence technologies have been the preferred method for detection, analytical sensing, medical diagnostics, biotechnology, imaging, and gene expression for many years. Fluorescence becomes essential for studying molecular processes with high specificity and sensitivity through a variety of biological processes. A significant problem for practical fluorescence applications is the apparent non-linearity of the fluorescence intensity resulting from inner-filter effects, sample scattering, and absorption of intrinsic components of biological samples. Sample absorption can lead to the primary inner filter effect (Type I inner filter effect) and is the first factor that should be considered. This is a relatively simple factor to be controlled in any fluorescence experiment. However, many previous approaches have given only approximate experimental methods for correcting the deviation from expected results. In this part we are discussing the origin of the primary inner filter effe...

中文翻译：

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关于荧光内滤光效果的起源和校正第一部分：主要内滤光效果-校正样品吸光度的正确方法。

多年来，荧光技术一直是检测，分析传感，医学诊断，生物技术，成像和基因表达的首选方法。荧光对于通过各种生物过程研究具有高特异性和敏感性的分子过程至关重要。实际荧光应用中的一个重要问题是荧光强度的明显非线性，这是由于内部滤光片效应，样品散射和生物样品内在成分吸收所导致的。样品吸收会导致主要的内部过滤器效应（I型内部过滤器效应），这是应首先考虑的因素。在任何荧光实验中，这都是要控制的相对简单的因素。然而，许多先前的方法仅给出了近似的实验方法来校正与预期结果的偏差。在这一部分中，我们将讨论主要内部过滤器效果的起源。