The goal of this study was to use 3D bioprinting technology to create a bioengineered dental construct containing human dental pulp stem cells (hDPSCs). To accomplish this, we first developed a novel bone morphogenetic protein (BMP) peptide-tethering bioink formulation and examined its rheological properties, its printability, and the structural stability of the bioprinted construct. Second, we evaluated the survival and differentiation of hDPSCs in the bioprinted dental construct by measuring cell viability, proliferation, and gene expression, as well as histological and immunofluorescent analyses. Our results showed that the peptide conjugation into the gelatin methacrylate-based bioink formulation was successfully performed. We determined that greater than 50% of the peptides remained in the bioprinted construct after three weeks in vitro cell culture. Human DPSC viability was >90% in the bioprinted constructs immediately after the printing process. Alizarin Red sta...

中文翻译：

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BMP 模拟肽束缚生物墨水对 3D 生物打印牙科结构中牙髓干细胞 (DPSC) 分化的影响。


本研究的目标是利用 3D 生物打印技术创建含有人牙髓干细胞 (hDPSC) 的生物工程牙科结构。为了实现这一目标，我们首先开发了一种新型骨形态发生蛋白（BMP）肽束缚生物墨水配方，并检查了其流变特性、可打印性以及生物打印构建体的结构稳定性。其次，我们通过测量细胞活力、增殖和基因表达以及组织学和免疫荧光分析来评估生物打印牙科结构中 hDPSC 的存活和分化。我们的结果表明，肽缀合到基于明胶甲基丙烯酸酯的生物墨水配方中已成功进行。我们确定，体外细胞培养三周后，超过 50% 的肽仍保留在生物打印的构建体中。打印过程结束后，生物打印结构中的人类 DPSC 活力>90%。茜素红...