There is increasing evidence that the expression of non-coding RNA and mRNA (messenger RNA) is significantly altered following high-dose ionizing radiation (IR), and their expression may play a critical role in cellular responses to IR. However, the role of non-coding RNA and mRNA in radiation protection, especially in the nervous system, remains unknown. In this study, microarray profiles were used to determine microRNA (miRNA), long non-coding RNA (lncRNA), and mRNA expression in the hypothalamus of mice that were pretreated with amifostine and subsequently exposed to high-dose IR. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed. We found that fewer miRNAs, lncRNAs, and mRNAs were induced by amifostine pre-treatment in exposed mice, which exhibited antagonistic effects compared to IR, indicating that amifostine attenuated the IR-induced effects on RNA profiles. GO and KEGG pathway analyses showed changes in a variety of signaling pathways involved in inflammatory responses during radioprotection following amifostine pre-treatment in exposed mice. Taken together, our study revealed that amifostine treatment altered or attenuated miRNA, lncRNA, and mRNA expression in the hypothalamus of exposed mice. These data provide a resource to further elucidate the mechanisms underlying amifostine-mediated radioprotection in the hypothalamus.

中文翻译：

---

氨磷汀预处理对暴露于60Coγ射线的小鼠下丘脑MIRNA，LNCRNA和MRNA谱的影响。

越来越多的证据表明，高剂量电离辐射（IR）后非编码RNA和mRNA（信使RNA）的表达会发生显着变化，并且它们的表达可能在细胞对IR的反应中起关键作用。然而，非编码RNA和mRNA在辐射防护中，尤其是在神经系统中的作用仍然未知。在这项研究中，微阵列概况用于确定经过氨磷汀预处理并随后暴露于大剂量IR的小鼠下丘脑中的microRNA（miRNA），长非编码RNA（lncRNA）和mRNA表达。进行了基因本体论（GO）和《京都议定书基因与基因组百科全书》（KEGG）途径分析。我们发现在暴露的小鼠中，氨磷汀预处理可诱导较少的miRNA，lncRNA和mRNA，与IR相比，它们具有拮抗作用，表明氨磷汀可减弱IR诱导的RNA谱图影响。GO和KEGG途径分析显示，在氨磷汀预处理后的暴露小鼠中，放射防护过程中炎症反应中涉及的多种信号传导途径发生了变化。两者合计，我们的研究表明，氨磷汀治疗改变或减弱了暴露小鼠下丘脑中的miRNA，lncRNA和mRNA表达。这些数据为进一步阐明下丘脑中氨磷汀介导的放射防护的机制提供了资源。我们的研究表明，氨磷汀治疗可改变或减弱暴露小鼠下丘脑中的miRNA，lncRNA和mRNA表达。这些数据为进一步阐明下丘脑中氨磷汀介导的放射防护的机制提供了资源。我们的研究表明，氨磷汀治疗可改变或减弱暴露小鼠下丘脑中的miRNA，lncRNA和mRNA表达。这些数据提供了进一步阐明下丘脑中氨磷汀介导的放射防护机制的资源。