Tobacco mosaic virus (TMV) infection can causes serious damage to tobacco crops. To explore the approach of preventing TMV infection of plants, two tobacco cultivars with different resistances to TMV were used to analyze transcription profiling before and after TMV infection. The involvement of biological pathways differed between the tolerant variety (Yuyan8) and the susceptible variety (NC89). In particular, the plant-virus interaction pathway was rapidly activated in Yuyan8, and specific resistance genes were enriched. Liquid chromatography tandem mass spectrometry analysis detected large quantities of antiviral substances in the tolerant Yuyan8. A novel Nicotiana tabacum leucine-rich repeat receptor kinase (NtLRR-RLK) gene was identified as being methylated and this was verified using bisulfite sequencing. Transient expression of TMV-green fluorescent protein in pRNAi-NtLRR-RLK transgenic plants confirmed that NtLRR-RLK was important for susceptibility to TMV. The specific protein interaction map generated from our study revealed that levels of BIP1, E3 ubiquitin ligase, and LRR-RLK were significantly elevated, and all were represented at node positions in the protein interaction map. The same expression tendency of these proteins was also found in pRNAi-NtLRR-RLK transgenic plants at 24 h after TMV inoculation. These data suggested that specific genes in the infection process can activate the immune signal cascade through different resistance genes, and the integration of signal pathways could produce resistance to the virus. These results contribute to the overall understanding of the molecular basis of plant resistance to TMV and in the long term could identify new strategies for prevention and control virus infection.

中文翻译：

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鉴定有助于烟草 TMV 耐受的新型 NtLRR-RLK 和生物途径。


烟草花叶病毒（TMV）感染会对烟草作物造成严重损害。为了探索预防植物TMV感染的方法，采用两个对TMV具有不同抗性的烟草品种来分析TMV感染前后的转录谱。耐受品种（Yuyan8）和感病品种（NC89）之间的生物学途径的参与不同。特别是，Yuyan8中植物-病毒相互作用途径被迅速激活，特异性抗性基因得到富集。液相色谱串联质谱分析在耐受的雨燕8号中检测到大量的抗病毒物质。一种新的烟草富含亮氨酸重复受体激酶 (NtLRR-RLK) 基因被鉴定为甲基化，并使用亚硫酸氢盐测序进行了验证。 TMV-绿色荧光蛋白在pRNAi-NtLRR-RLK转基因植物中的瞬时表达证实了NtLRR-RLK对于TMV的易感性很重要。我们的研究生成的特定蛋白质相互作用图显示，BIP1、E3 泛素连接酶和 LRR-RLK 的水平显着升高，并且全部都出现在蛋白质相互作用图的节点位置。在TMV接种后24小时，在pRNAi-NtLRR-RLK转基因植物中也发现了这些蛋白的相同表达趋势。这些数据表明，感染过程中的特定基因可以通过不同的抗性基因激活免疫信号级联，信号通路的整合可以产生对病毒的抗性。这些结果有助于全面了解植物对 TMV 抗性的分子基础，从长远来看，可以确定预防和控制病毒感染的新策略。