BACKGROUND Peanut allergy is the most prevalent and dangerous food allergy. Peanuts consist of a large number of different allergens and peanut-allergic patients are frequently sensitized to multiple allergens. Hence, conventional desensitization approaches aim at targeting as many allergens as possible. METHODS The monoclonal anti-Ara h 2 antibody (mAb) was produced by hybridoma cells derived from WT BALB/c mice after immunization with a vaccine based on virus-like particles coupled to Ara h 2. BALB/c mice were sensitized intraperitoneally with peanut extract absorbed to alum and mAbs were applied i.v. Challenge was performed the next day with the whole peanut extract intravenously and via skin prick test. RESULTS Here we show in peanut-allergic mice that a single high-affinity mAb specific for Ara h 2 is able to block systemic and local allergic reactions induced by the complex peanut extract. We confirm in vitro binding of the mAb to the inhibitory low-affinity FcγRIIb receptor using a sensitive biosensor and demonstrate in vivo that protection was dependent on FcγRIIb. CONCLUSION A single mAb specific for Ara h 2 is able to improve local and systemic allergic symptoms induced by the whole allergen mixture.

中文翻译：

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抗花生过敏原 Ara h 2 的单克隆抗体可预防全身和局部花生过敏。


背景花生过敏是最普遍和最危险的食物过敏。花生含有大量不同的过敏原，花生过敏患者经常对多种过敏原过敏。因此，传统的脱敏方法旨在针对尽可能多的过敏原。方法 使用基于与 Ara h 2 偶联的病毒样颗粒的疫苗进行免疫后，由 WT BALB/c 小鼠衍生的杂交瘤细胞产生单克隆抗 Ara h 2 抗体 (mAb)。 BALB/c 小鼠腹膜内注射花生致敏吸收到明矾的提取物和单克隆抗体被静脉注射，第二天用全花生提取物静脉注射并通过皮肤点刺试验进行挑战。结果在这里，我们在花生过敏小鼠中证明，针对 Ara h 2 的单一高亲和力单克隆抗体能够阻断由复合花生提取物引起的全身和局部过敏反应。我们使用灵敏的生物传感器在体外证实了 mAb 与抑制性低亲和力 FcγRIIb 受体的结合，并在体内证明了这种保护依赖于 FcγRIIb。结论 单一针对 Ara h 2 的单克隆抗体能够改善由整个过敏原混合物引起的局部和全身过敏症状。