In comparison to the extensive characterization of haemagglutinin antibodies of avian influenza virus (AIV), the role of neuraminidase (NA) as an immunogen is less well understood. This study describes the construction and cellular responses of recombinant fowlpox viruses (rFWPV) strain FP9, co-expressing NA N1 gene of AIV A/Chicken/Malaysia/5858/2004, and chicken IL-12 gene. Our data shows that the N1 and IL-12 proteins were successfully expressed from the recombinants with 48 kD and 70 kD molecular weights, respectively. Upon inoculation into specific-pathogen-free (SPF) chickens at 105 p.f.u. ml−1, levels of CD3+/CD4+ and CD3+/CD8+ populations were higher in the wild-type fowlpox virus FP9 strain, compared to those of rFWPV-N1 and rFWPV-N1-IL-12 at weeks 2 and 5 time points. Furthermore, rFWPV-N1-IL-12 showed a suppressive effect on chicken body weight within 4 weeks after inoculation. We suggest that co-expression of N1 with or without IL-12 offers undesirable quality as a potential AIV vaccine candidate.

中文翻译：

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鸡细胞因子IL-12整合入表达禽流感病毒神经氨酸酶N1的重组鸡痘病毒对宿主细胞免疫反应的影响可忽略不计。

与禽流感病毒（AIV）的血凝素抗体的广泛表征相比，人们对神经氨酸酶（NA）作为免疫原的作用了解较少。这项研究描述了重组禽痘病毒（rFWPV）株FP9，共表达AIV A / Chicken / Malaysia / 5858/2004的NA N1基因和鸡IL-12基因的构建和细胞应答。我们的数据显示，分别从分子量为48 kD和70 kD的重组体成功表达了N1和IL-12蛋白。接种到10 5 pfu ml -1的无特定病原（SPF）的鸡中时在第2周和第5个时间点，与rFWPV-N1和rFWPV-N1-IL-12相比，野生型鸡痘病毒FP9株中CD3 + / CD4 +和CD3 + / CD8 +种群的水平更高。此外，rFWPV-N1-IL-12在接种后4周内显示出对鸡体重的抑制作用。我们建议将N1与或不与IL-12共表达可作为潜在的AIV疫苗候选物提供不良质量。