In comparison to the extensive characterization of haemagglutinin antibodies of avian influenza virus (AIV), the role of neuraminidase (NA) as an immunogen is less well understood. This study describes the construction and cellular responses of recombinant fowlpox viruses (rFWPV) strain FP9, co-expressing NA N1 gene of AIV A/Chicken/Malaysia/5858/2004, and chicken IL-12 gene. Our data shows that the N1 and IL-12 proteins were successfully expressed from the recombinants with 48 kD and 70 kD molecular weights, respectively. Upon inoculation into specific-pathogen-free (SPF) chickens at 105 p.f.u. ml−1, levels of CD3+/CD4+ and CD3+/CD8+ populations were higher in the wild-type fowlpox virus FP9 strain, compared to those of rFWPV-N1 and rFWPV-N1-IL-12 at weeks 2 and 5 time points. Furthermore, rFWPV-N1-IL-12 showed a suppressive effect on chicken body weight within 4 weeks after inoculation. We suggest that co-expression of N1 with or without IL-12 offers undesirable quality as a potential AIV vaccine candidate.

中文翻译：

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鸡细胞因子 IL-12 整合到表达禽流感病毒神经氨酸酶 N1 的重组鸡痘病毒中对宿主细胞免疫反应的影响可以忽略不计。


与禽流感病毒（AIV）血凝素抗体的广泛表征相比，神经氨酸酶（NA）作为免疫原的作用尚不清楚。本研究描述了重组鸡痘病毒 (rFWPV) FP9 株的构建和细胞反应，该病毒株共表达 AIV A/Chicken/Malaysia/5858/2004 的 NA N1 基因和鸡 IL-12 基因。我们的数据显示，重组体成功表达了 N1 和 IL-12 蛋白，分子量分别为 48 kD 和 70 kD。以 10 5 pfu ml -1接种到无特定病原体 (SPF) 鸡后，与 rFWPV-N1 和 rFWPV-N1 相比，野生型鸡痘病毒 FP9 株中 CD3+/CD4+ 和 CD3+/CD8+ 群体的水平较高。 rFWPV-N1-IL-12 在第 2 周和第 5 周时间点。此外，rFWPV-N1-IL-12在接种后4周内显示出对鸡体重的抑制作用。我们认为，N1 与或不与 IL-12 共表达作为潜在的 AIV 候选疫苗的质量不理想。