Introduction. Sporotrichosis, caused by species of the Sporothrix schenckii complex, is the most prevalent subcutaneous mycosis in many areas of Latin America. Statins are a class of drugs widely used for lowering high sterol levels through their action on 3-hydroxy-3-methylglutaryl-CoA reductase, a key enzyme in the synthesis of sterol. Aim. In this study, the antifungal activity of statins (simvastatin, atorvastatin, pravastatin) against planktonic cells and biofilms of S. schenckii complex species was evaluated, as well as the interaction of pravastatin with classical antifungals (amphotericin B, itraconazole, terbinafine). Methodology. Eighteen strains of Sporothrix species were used. The antifungal susceptibility assay was performed using the broth microdilution method. Mature biofilms were exposed to statins and metabolic activity was measured by the XTT reduction assay. Results. MICs of statins ranged from 8 to 512 μg ml−1 and from 8 to 256 μg ml−1 for filamentous and yeast forms, respectively. Regarding mature biofilms, MICs of 50 % inhibition (SMIC50) were 128 μg ml−1 for simvastatin and atorvastatin and >2048 μg ml−1 for pravastatin. MICs of 90 % inhibition (SMIC90) were 512 μg ml−1 for simvastatin and >2048 μg ml−1 for atorvastatin and pravastatin. Conclusion. These results highlight the antifungal and antibiofilm potential of statins against S. schenckii complex species.

中文翻译：

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他汀类药物对Sporothrix schenckii物种复合体的浮游细胞和生物膜的体外抑制作用。

介绍。由Sporothrix schenckii复合物的种类引起的孢子菌病是拉丁美洲许多地区最普遍的皮下真菌病。他汀类药物是一类药物，通过作用于3-羟基-3-甲基戊二酰辅酶A还原酶（甾醇合成中的关键酶）而广泛用于降低高固醇水平。目标。在这项研究中，他汀类药物（辛伐他汀，阿托伐他汀，普伐他汀）对S. schenckii复杂物种的浮游细胞和生物膜的抗真菌活性以及普伐他汀与经典抗真菌药（两性霉素B，伊曲康唑，特比萘芬）的相互作用进行了评估。方法。十八孢子菌物种被使用。使用肉汤微量稀释法进行抗真菌药敏试验。将成熟的生物膜暴露于他汀类药物，并通过XTT还原测定法测量代谢活性。结果。对于丝状和酵母形式，他汀类药物的MIC分别为8至512μgml -1和8至256μgml -1。关于成熟生物膜，50％抑制（SMIC50）的MIC值分别为128微克毫升-1为辛伐他汀和阿托伐他汀和> 2048微克毫升-1普伐他汀。90％抑制（SMIC90）的MIC值分别为512微克毫升-1为辛伐他汀和> 2048微克毫升-1为阿托伐他汀和普伐他汀。结论。这些结果突出了他汀类药物对S. schenckii复杂物种的抗真菌和抗生物膜的潜力。