Background: NN-32 toxin, which was obtained from Naja naja venom and showed cytotoxicity on cancer cell lines. As the toxicity of NN-32 is the main hurdle in the process of drug development; hence, we have conjugated NN-32 toxin with gold nanoparticles (GNP-NN-32) in order to decrease the toxicity of NN-32 without reducing its efficacy, GNP-NN-32 alleviated the toxicity of NN-32 in in vitro studies during the course of earlier studies. In continuation, we are evaluating in vivo toxicity profile of NN-32 and GNP-NN-32 in the present study.

Objective: To study in vivo toxicity profile of NN-32 and nanogold conjugated GNP-NN-32 from Naja naja venom.

Materials and Methods: We have carried out in vivo acute toxicity study to determine LD50 dose of GNP-NN-32, in vivo sub-chronic toxicity for 30 days, haematology, serum biochemical parameters and histopathology study on various mice tissues and in vitro cellular and tissue toxicity studies.

Results: The LD50 dose of GNP-NN-32 was found to be 2.58 mg/kg (i.p.) in Swiss male albino mice. In vivo sub-chronic toxicity showed significantly reduced toxicity of GNP-NN-32 as compared to NN-32 alone.

Discussion: In vitro cellular toxicity studies on human lymphocyte and mouse peritoneal macrophage showed significant inhibition of cells by NN-32 alone.

Conclusion: Conjugated GNP-NN-32 toxin showed less in vivo toxicity as compared to pure NN-32.

背景：NN-32 毒素，从 Naja naja 毒液中获得，对癌细胞系具有细胞毒性。由于NN-32的毒性是药物开发过程中的主要障碍；因此，我们将 NN-32 毒素与金纳米粒子 (GNP-NN-32) 结合以降低 NN-32 的毒性而不降低其功效，GNP-NN-32 在体外研究中减轻了 NN-32 的毒性在早期的学习过程中。继续，我们正在评估本研究中 NN-32 和 GNP-NN-32 的体内毒性特征。

目的：研究 NN-32 和纳米金缀合的 GNP-NN-32 来自 Naja Naja 毒液的体内毒性特征。

材料和方法：我们进行了体内急性毒性研究，以确定 GNP-NN-32 的 LD50 剂量，体内 30 天的亚慢性毒性，血液学、血清生化参数和各种小鼠组织和体外细胞的组织病理学研究。和组织毒性研究。

结果：在瑞士雄性白化小鼠中发现 GNP-NN-32 的 LD50 剂量为 2.58 mg/kg (ip)。与单独的 NN-32 相比，体内亚慢性毒性表明 GNP-NN-32 的毒性显着降低。

讨论：对人淋巴细胞和小鼠腹腔巨噬细胞的体外细胞毒性研究表明，单独使用 NN-32 可显着抑制细胞。

结论：与纯 NN-32 相比，缀合的 GNP-NN-32 毒素显示出较低的体内毒性。