当前位置：
X-MOL 学术
›
J. Inflammation Res.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Impact of Low Interferon-γ and IL-10 Levels on TNF-α and IL-6 Production by PHA-Induced PBMCs in Type 2 Diabetes Mellitus.
Journal of Inflammation Research ( IF 4.2 ) Pub Date : 2020-04-22 , DOI: 10.2147/jir.s245064 Rona Kartika 1 , Dyah Purnamasari 2 , Saraswati Pradipta 1 , Rahma A Larasati 3 , Heri Wibowo 4
Journal of Inflammation Research ( IF 4.2 ) Pub Date : 2020-04-22 , DOI: 10.2147/jir.s245064 Rona Kartika 1 , Dyah Purnamasari 2 , Saraswati Pradipta 1 , Rahma A Larasati 3 , Heri Wibowo 4
Affiliation
Purpose: In this study, we analyzed the production of interferon γ (IFN-γ) and interleukin 10 (IL-10) by peripheral blood mononuclear cells (PBMCs) in patients with type 2 diabetes mellitus (T2DM). We aimed to investigate the capacity of monocytes to produce tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) following IFN-γ stimulation and the associated role of IL-10 in TNF-α and IL-6 production.
Patients and Methods: In vitro experiments were conducted on PBMCs obtained from 19 patients with T2DM and 17 healthy participants. PBMCs were isolated from venous blood by density gradient centrifugation, followed by 3-day phytohemagglutinin induction. In vitro production of TNF-α, IL-6, IFN-γ, and IL-10 was measured using the multiplex immunoassay. Statistical analysis was performed using IBM SPSS 23 version.
Results: IFN-γ concentration in the T2DM group was significantly lower than that in control group (T2DM 7,700.86 ± 3,037.77 vs control 10,672.69 ± 5,625.50 pg/mL; p = 0.048). However, TNF-α, IL-6, and IL-10 levels showed no significant difference between the two groups. The TNF-α/IFN-γ and IL-6/IFN-γ ratios were significantly higher in T2DM than in the control group (p = 0.026 and p = 0.048, respectively). In T2DM, the high TNF-α/IFN-γ ratio was consistent, with the low baseline IL-10 level (p = 0.022).
Conclusion: In T2DM, T-cell response is impaired with significantly reduced IFN-γ production, and simultaneously, circulatory monocytes show enhanced cellular responsiveness to inflammatory stimuli. The low baseline IL-10 level likely contributes to such an immune response.
Keywords: type 2 diabetes mellitus, proinflammatory response, cytokines
中文翻译：
低干扰素-γ 和 IL-10 水平对 2 型糖尿病中 PHA 诱导的 PBMC 产生 TNF-α 和 IL-6 的影响。
目的：在本研究中，我们分析了 2 型糖尿病 (T2DM) 患者外周血单个核细胞 (PBMC) 产生的干扰素 γ (IFN-γ) 和白细胞介素 10 (IL-10)。我们旨在研究 IFN-γ 刺激后单核细胞产生肿瘤坏死因子-α (TNF-α) 和白细胞介素 6 (IL-6) 的能力以及 IL-10 在 TNF-α 和 IL-6 产生中的相关作用.
患者和方法：对从 19 名 T2DM 患者和 17 名健康参与者获得的 PBMC 进行了体外实验。通过密度梯度离心从静脉血中分离 PBMC，然后进行 3 天植物血凝素诱导。使用多重免疫测定法测量 TNF-α、IL-6、IFN-γ 和 IL-10 的体外产生。使用 IBM SPSS 23 版本进行统计分析。
结果： T2DM 组的 IFN-γ 浓度显着低于对照组（T2DM 7,700.86 ± 3,037.77 vs 对照组 10,672.69 ± 5,625.50 pg/mL；p = 0.048）。然而，TNF-α、IL-6和IL-10水平在两组之间没有显着差异。T2DM 患者的 TNF-α/IFN-γ 和 IL-6/IFN-γ 比值显着高于对照组（p= 0.026 和p = 0.048，分别）。在 T2DM 中，高 TNF-α/IFN-γ 比率与基线 IL-10 水平低（p = 0.022）一致。
结论：在 T2DM 中，T 细胞反应受损，IFN-γ 产生显着减少，同时循环单核细胞对炎症刺激的反应性增强。低基线 IL-10 水平可能有助于这种免疫反应。
【关键词】： 2型糖尿病 促炎反应 细胞因子
更新日期：2020-04-22
Patients and Methods: In vitro experiments were conducted on PBMCs obtained from 19 patients with T2DM and 17 healthy participants. PBMCs were isolated from venous blood by density gradient centrifugation, followed by 3-day phytohemagglutinin induction. In vitro production of TNF-α, IL-6, IFN-γ, and IL-10 was measured using the multiplex immunoassay. Statistical analysis was performed using IBM SPSS 23 version.
Results: IFN-γ concentration in the T2DM group was significantly lower than that in control group (T2DM 7,700.86 ± 3,037.77 vs control 10,672.69 ± 5,625.50 pg/mL; p = 0.048). However, TNF-α, IL-6, and IL-10 levels showed no significant difference between the two groups. The TNF-α/IFN-γ and IL-6/IFN-γ ratios were significantly higher in T2DM than in the control group (p = 0.026 and p = 0.048, respectively). In T2DM, the high TNF-α/IFN-γ ratio was consistent, with the low baseline IL-10 level (p = 0.022).
Conclusion: In T2DM, T-cell response is impaired with significantly reduced IFN-γ production, and simultaneously, circulatory monocytes show enhanced cellular responsiveness to inflammatory stimuli. The low baseline IL-10 level likely contributes to such an immune response.
Keywords: type 2 diabetes mellitus, proinflammatory response, cytokines
中文翻译：
低干扰素-γ 和 IL-10 水平对 2 型糖尿病中 PHA 诱导的 PBMC 产生 TNF-α 和 IL-6 的影响。
目的：在本研究中，我们分析了 2 型糖尿病 (T2DM) 患者外周血单个核细胞 (PBMC) 产生的干扰素 γ (IFN-γ) 和白细胞介素 10 (IL-10)。我们旨在研究 IFN-γ 刺激后单核细胞产生肿瘤坏死因子-α (TNF-α) 和白细胞介素 6 (IL-6) 的能力以及 IL-10 在 TNF-α 和 IL-6 产生中的相关作用.
患者和方法：对从 19 名 T2DM 患者和 17 名健康参与者获得的 PBMC 进行了体外实验。通过密度梯度离心从静脉血中分离 PBMC，然后进行 3 天植物血凝素诱导。使用多重免疫测定法测量 TNF-α、IL-6、IFN-γ 和 IL-10 的体外产生。使用 IBM SPSS 23 版本进行统计分析。
结果： T2DM 组的 IFN-γ 浓度显着低于对照组（T2DM 7,700.86 ± 3,037.77 vs 对照组 10,672.69 ± 5,625.50 pg/mL；p = 0.048）。然而，TNF-α、IL-6和IL-10水平在两组之间没有显着差异。T2DM 患者的 TNF-α/IFN-γ 和 IL-6/IFN-γ 比值显着高于对照组（p= 0.026 和p = 0.048，分别）。在 T2DM 中，高 TNF-α/IFN-γ 比率与基线 IL-10 水平低（p = 0.022）一致。
结论：在 T2DM 中，T 细胞反应受损，IFN-γ 产生显着减少，同时循环单核细胞对炎症刺激的反应性增强。低基线 IL-10 水平可能有助于这种免疫反应。
【关键词】： 2型糖尿病 促炎反应 细胞因子
京公网安备 11010802027423号