Lipids such as cholesterol, triacylglycerols, and fatty acids play important roles in the regulation of cellular metabolism and cellular signaling pathways and, as a consequence, in the development of various diseases. It is therefore important to understand how their metabolism is regulated to better define the components involved in the development of various human diseases. In the present work, we describe the development and validation of a high‐performance thin layer chromatography (HPTLC) method allowing the separation and quantification of free cholesterol, cholesteryl esters, nonesterified fatty acids, and triacylglycerols. This method will be of interest as the quantification of these lipids in one single assay is difficult to perform.

中文翻译：

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一种新型的基于高效薄层色谱的方法，用于同时定量测定来自细胞和组织提取物的临床相关脂质。

诸如胆固醇，三酰基甘油和脂肪酸之类的脂质在细胞代谢和细胞信号传导途径的调节中以及因此在各种疾病的发展中起重要作用。因此，重要的是要了解如何调节其新陈代谢，以更好地定义各种人类疾病的发展所涉及的成分。在当前的工作中，我们描述了高性能薄层色谱（HPTLC）方法的开发和验证，该方法允许分离和定量游离胆固醇，胆固醇酯，非酯化脂肪酸和三酰基甘油。由于难以在单个测定中对这些脂质进行定量，因此该方法将引起人们的兴趣。