当前位置: X-MOL 学术Int. Arch. Allergy Immunol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
IL-15 Expression Pattern in Atopic Dermatitis.
International Archives of Allergy and Immunology ( IF 2.5 ) Pub Date : 2020-05-18 , DOI: 10.1159/000508515
Hélène Karlen 1 , Shida Yousefi 2 , Hans-Uwe Simon 2, 3 , Dagmar Simon 4
Affiliation  

Background: An increased expression of interleukin (IL)-15, a cytokine with a key role in stimulating innate and adaptive immune cells, such as dendritic cells (DC), natural killer cells, and T cells, has been observed in infectious and inflammatory diseases, including autoimmune diseases as well as cancer. Atopic dermatitis (AD) is a common inflammatory skin disease characterized by a type 2 immune response. Objective: To explore the expression of IL-15 and its pattern in AD skin. Method: Immunofluorescence staining was performed on skin specimens of AD skin, nonlesional AD skin (AD NL), and normal skin (NS) using antibodies directed against IL-15 and CD3, mast cell tryptase, eosinophil cationic protein, CD68, CD11b, CD1a, and vimentin. Results: A significantly higher IL-15 expression in AD and AD NL was observed in both the epidermis (p = 0.0003) and the dermis (p = 0.0154) as compared to NS. Cells expressing IL-15 were mainly keratinocytes, CD1a+ DC, CD11b+ DC, CD68+ macrophages, and vimentin+ fibroblasts. In AD, an increase in the relative numbers of IL-15 expressing CD1a+ DC, macrophages, and fibroblasts was noted. Conclusion: Our results demonstrate an expression of IL-15 in AD similar to that of eosinophilic esophagitis which is also a type 2 disease. IL-15 may serve as a therapeutic target for AD.
Int Arch Allergy Immunol


中文翻译:

特应性皮炎中的IL-15表达模式。

背景:白细胞介素(IL)-15的表达增加,这种细胞因子在刺激先天性和适应性免疫细胞(如树突状细胞(DC),自然杀伤细胞和T细胞)中起关键作用疾病,包括自身免疫性疾病以及癌症。特应性皮炎(AD)是一种常见的炎症性皮肤病,其特征在于2型免疫反应。目的:探讨IL-15在AD皮肤中的表达及其模式。方法:使用针对IL-15和CD3,肥大细胞类胰蛋白酶,嗜酸性粒细胞阳离子蛋白,CD68,CD11b,CD1a的抗体,对AD皮肤,非病变性AD皮肤(AD NL)和正常皮肤(NS)的皮肤样本进行免疫荧光染色。和波形蛋白。结果:与NS相比,在表皮(p = 0.0003)和真皮(p = 0.0154)中都观察到AD和AD NL中IL-15的表达明显升高。表达IL-15的细胞主要是角质形成细胞,CD1a + DC,CD11b + DC,CD68 +巨噬细胞和波形蛋白+成纤维细胞。在AD中,注意到表达IL-15的CD1a + DC,巨噬细胞和成纤维细胞的相对数量增加。结论:我们的结果证明IL-15在AD中的表达与嗜酸性食管炎相似,后者也是2型疾病。IL-15可以作为AD的治疗靶标。
Int Arch过敏免疫
更新日期:2020-05-18
down
wechat
bug