Over the past five years, technological advances have made it possible to image the translation of single mRNA in the natural context of living cells. With these advances, researchers are beginning to shed light on when, where, and to what degree mRNA are translated with single-molecule precision. These works provide insight into the heterogeneity of translation amongst single transcripts, behavior that is averaged out in complementary bulk assays. In this review, we discuss the rapidly maturing field of live-cell, single-mRNA imaging of translation, beginning with a brief overview of recent technological advances. The remainder of the review focuses on the new biological insights gained from these technologies. We conclude with a discussion of the future of this technology.

中文翻译：

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点亮活细胞中的单 mRNA 翻译动态。


过去五年来，技术进步使得在活细胞的自然环境中对单个 mRNA 的翻译进行成像成为可能。凭借这些进展，研究人员开始揭示 mRNA 在何时、何地以及在何种程度上以单分子精度进行翻译。这些工作提供了对单个转录本之间翻译异质性的深入了解，以及在互补批量分析中平均的行为。在这篇综述中，我们首先简要概述了最新的技术进展，讨论了快速成熟的活细胞、单 mRNA 翻译成像领域。评论的其余部分重点关注从这些技术中获得的新的生物学见解。最后我们讨论了这项技术的未来。