Generating novel functionality from well characterised synthetic parts and modules lies at the heart of synthetic biology. Ideally, circuitry is rationally designed in silico with quantitatively predictive models to predetermined design specifications. Synthetic circuits are intrinsically stochastic, often dynamically modulated and set in a dynamic fluctuating environment within a living cell. To build more complex circuits and to gain insight into context effects, intrinsic noise and transient performance, characterisation techniques that resolve both heterogeneity and dynamics are required. Here we review recent advances in both in vitro and in vivo microfluidic technologies that are suitable for the characterisation of synthetic circuitry, modules and parts.

中文翻译：

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用于合成电路动态表征的微流体平台。

从特征明确的合成零件和模块中产生新颖的功能是合成生物学的核心。理想情况下，采用定量预测模型对电路进行合理的计算机设计，以达到预定的设计规格。合成电路本质上是随机的，通常是动态调制的，并设置在活细胞内动态变化的环境中。为了构建更复杂的电路并深入了解环境影响，固有噪声和瞬态性能，需要解决异质性和动力学特性的表征技术。在这里，我们回顾了适用于表征合成电路，模块和零件的体外和体内微流体技术的最新进展。