Genetically encodable fluorescent biosensors provide spatiotemporal information on their target analytes in a label-free manner, which has enabled the study of cell biology and signaling in living cells. Over the past three decades, fueled by the development of a wide palette of fluorescent proteins, protein-based fluorescent biosensors against a broad array of targets have been developed. Recently, with the development of fluorogenic RNA aptamer-dye pairs that function in live cells, RNA-based fluorescent (RBF) biosensors have emerged as a complementary class of biosensors. Here we review the current state-of-the-art for fluorogenic RNA aptamers and RBF biosensors for imaging small molecules and RNAs, and highlight some emerging opportunities.

中文翻译：

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基于RNA的荧光生物传感器，用于小分子和RNA的活细胞成像。

可遗传编码的荧光生物传感器以无标记的方式提供其靶标分析物的时空信息，这使得能够研究活细胞中的细胞生物学和信号传导。在过去的三十年中，在开发出广泛的荧光蛋白调色板的推动下，已经开发出了针对多种靶标的基于蛋白质的荧光生物传感器。近来，随着在活细胞中起作用的荧光RNA适体染料对的发展，基于RNA的荧光（RBF）生物传感器已成为生物传感器的互补类。在这里，我们回顾了用于成像小分子和RNA的荧光RNA适体和RBF生物传感器的最新技术，并重点介绍了一些新兴机会。