Background/Aims

CircABCB10 function as an endogenous miRNA sponge plays an important role in various tumors. This experimental design was based on circABCB10 to explore the pathogenesis of non-small cell lung cancer (NSCLC). Methods: CircRNA microarray was used to examine circRNA expression profiles in lung cancer from 3 NSCLC patients and paired healthy lung tissues. The expression of circABCB10 and miR-584-5p was detected by q-PCR. CCK-8, colony formation, and transwell assays to study the circABCB10 effects on tumor cell growth and cell migration invasiveness. To validate downstream target genes of circABCB10 and miR-584-5p detected by luciferase reporter assays. RT-qPCR and Western blotting were used to study E2F5 expression. The tumor growth was detected by nude mice in vivo. Results: We analyzed the human circRNA expression profile in NSCLC tissues. CircABCB10 was identified as a circRNA that increased in NSCLC tissues. CircABCB10 was noticeably raised in NSCLC, and high circABCB10 expression was related to low survival in NSCLC patients. Silencing of circABCB10 suppressed non-small cell lung cancer cell migration, cell proliferation, and invasion.CircABCB10 can act as a sponge of miR-584-5p to up-regulate E2F5 expression level. E2F5 knockdown or overexpress of miR-584-5p gene reversed the circABCB10 who has carcinogenic effects. There was a negative correlation expression between the circABCB10 and miR-584-5p gene, and There was a positive relationship between the expression of circABCB10 and E2F5 in NSCLC tumors. Conclusion: CircABCB10 promoted the progression of NSCLC by modulating the miR-584-5p/E2F5 axis.

Abbreviation

NSCLC: non-small cell lung cancer; circ RNA: circular RNA; miRNA: micro RNA

中文翻译：

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环状 RNA ABCB10 通过海绵 miR-584-5p 增加 E2F5 表达，从而促进非小细胞肺癌进展。

 背景/目标

CircABCB10作为内源性miRNA海绵，在多种肿瘤中发挥着重要作用。本实验设计基于circABCB10来探讨非小细胞肺癌（NSCLC）的发病机制。方法：使用 CircRNA 微阵列检测 3 名 NSCLC 患者和配对健康肺组织的肺癌中的 circRNA 表达谱。 q-PCR检测circABCB10和miR-584-5p的表达。通过 CCK-8、集落形成和 Transwell 实验来研究 circABCB10 对肿瘤细胞生长和细胞迁移侵袭力的影响。验证荧光素酶报告基因检测检测到的 circABCB10 和 miR-584-5p 下游靶基因。 RT-qPCR 和蛋白质印迹用于研究 E2F5 表达。裸鼠体内检测肿瘤生长情况。结果：我们分析了 NSCLC 组织中的人类 circRNA 表达谱。 CircABCB10 被鉴定为在 NSCLC 组织中增加的 circRNA。 CircABCB10 在 NSCLC 中显着升高，并且 circABCB10 高表达与 NSCLC 患者的低生存率相关。 circABCB10的沉默抑制了非小细胞肺癌细胞的迁移、细胞增殖和侵袭。CircABCB10可以作为miR-584-5p的海绵来上调E2F5的表达水平。 E2F5敲低或过度表达miR-584-5p基因可逆转具有致癌作用的circABCB10。 NSCLC肿瘤中circABCB10与miR-584-5p基因的表达呈负相关，而circABCB10与E2F5的表达呈正相关。结论：CircABCB10通过调节miR-584-5p/E2F5轴促进NSCLC的进展。

 缩写

NSCLC：非小细胞肺癌； circ RNA：环状RNA； miRNA：微小RNA