We report the efforts to construct active targeting quantum dots using receptor-binding peptide for enhanced detection and migration inhibition of cancer cells. Peptide E5 has specific binding with chemokine receptor 4 (CXCR4), which is a transmembrane G-coupled receptor involved in the metastasis of various types of cancers. E5 was introduced to the surface of CdSe/ZnS quantum dots via biotin-streptavidin interactions. The constructed CXCR4-targeting quantum dots (E5@QDs) was observed to display improved detection sensitivity and significantly enhanced binding affinity for CXCR4 over-expressed cancer cells, and the ability to inhibit cancer cells migration induced by CXCL12.

我们报告了使用受体结合肽构建主动靶向量子点以增强对癌细胞的检测和迁移抑制的努力。肽 E5 与趋化因子受体 4 (CXCR4) 具有特异性结合，CXCR4 是一种跨膜 G 偶联受体，参与多种癌症的转移。通过生物素-链霉亲和素相互作用将 E5 引入 CdSe/ZnS 量子点的表面。观察到构建的 CXCR4 靶向量子点（E5@QDs）显示出提高的检测灵敏度和显着增强的对 CXCR4 过表达癌细胞的结合亲和力，以及抑制 CXCL12 诱导的癌细胞迁移的能力。