The ydcI gene has previously been shown to encode a DNA-binding protein involved with acid stress resistance and induced biofilm formation in a strain of Salmonella enterica serovar Typhimurium. In addition, characterisation of the ydcI gene in Escherichia coli and other bacteria demonstrated strikingly different tolerance for induced ydcI expression across Gram negative species. In this report, we investigated the conservation of these phenotypes across multiple strains of S. Typhimurium and E. coli, and we used RNA Seq to identify the transcriptome of the ΔydcI mutant compared to WT in S. Typhimurium and E. coli (to establish the YdcI regulon in each species). We constructed deletion mutants in each species based on the RNA Seq results and tested these mutants for the relevant ydcI-related phenotypes. Though no evidence for a role in these phenotypes was found via the RNA Seq deletion mutants, we found that the ydcI-induced biofilm in S. Typhimurium is formed independently of the major biofilm genes csgA and bcsA indicating a potentially novel type of biofilm formation.

所述ydcI基因先前已表明编码参与酸胁迫抗性和诱导生物膜形成一个DNA结合蛋白中的一个应变肠沙门氏菌鼠伤寒沙门氏菌。此外，在大肠杆菌和其他细菌中ydcI基因的表征证明，革兰氏阴性菌对ydcI表达的耐受性显着不同。在本报告中，我们调查了跨越多株这些表型的保护S.鼠伤寒沙门氏菌和大肠杆菌（E.coli） ，和我们使用RNA测序识别Δ的转录ydcI突变体与WT相比S.鼠伤寒和大肠杆菌（在每个物种中建立YdcI调节子）。我们基于RNA Seq结果在每个物种中构建了缺失突变体，并测试了这些突变体的相关ydcI相关表型。虽然在这些表型作用没有证据经由核糖核酸SEQ缺失突变体中发现，我们发现，ydcI中诱导生物膜S.伤寒独立于主要生物膜基因形成csgA和BCSA指示存在潜在的新类型的生物膜形成。