Chattonella spp. are known to produce large amounts of reactive oxygen species (ROS); however, little is known about the mechanisms involved in mitigating the intracellular accumulation of ROS. In this study, a time-series of biological responses in C. marina var. antiqua under different oxidative stress conditions, induced by adding H₂O₂ at the initial concentrations of 100 and 500 µM, was investigated. Although the added exogenous H₂O₂ was rapidly consumed at 3 h post-exposure (hpe), intracellular ROS levels were enhanced in the 500 µM H₂O₂ group but decreased in the 100 µM H₂O₂ group. Accompanied by increased intracellular ROS levels, the photosynthetic activity of C. marina var. antiqua was considerably inhibited in the 500 µM H₂O₂ group, but not in the 100 µM H₂O₂ group. The F_v/F_m ratio and PI_ABS were negatively correlated with the intracellular ROS level, while the ABS/RC, TR₀/RC, and DI₀/RC were positively correlated with the intracellular ROS level. Expression of the gene encoding 2-cysteine peroxiredoxin (2-Cys Prx) was up-regulated in 100 µM H₂O₂ group at 6 hpe, but was down-regulated in 100 µM H₂O₂ group at 3 and 6 hpe. A negative relationship between the 2-Cys Prx transcript levels and intracellular ROS levels was detected. Results of the 2-DE proteomic analysis confirmed that the 500 µM H₂O₂ treatment down-regulated the expression of 2-Cys Prx and induced more damage to photosynthetic abilities of C. marina var. antiqua.

查通氏菌属 已知会产生大量的活性氧（ROS）；然而，关于减轻细胞内ROS积累的机制知之甚少。在这项研究中，C。marina var。的生物反应的时间序列。研究了在100和500 µM初始浓度下加入H ₂ O ₂诱导的在不同氧化应激条件下的antiqua。尽管添加的外源H ₂ O ₂在接触后3 h（hpe）迅速消耗，但500 µM H ₂ O ₂组的细胞内ROS水平升高，而100 µM H ₂ O ₂组则降低组。伴随着细胞内ROS水平的提高，C。marina var的光合活性。在500 µM H ₂ O ₂组中antiqua被显着抑制，而在100 µM H ₂ O ₂组中则没有。F _v / F _m比和PI _ABS与细胞内ROS水平呈负相关，而ABS / RC，TR ₀ / RC和DI ₀ / RC与细胞内ROS水平呈正相关。在100 µM H ₂ O _2中上调了2-半胱氨酸过氧化物酶（2-Cys Prx）的编码基因的表达。组在6 hpe时，但在100μMH ₂ O ₂组中在3 hpe和6 hpe下调。检测到2-Cys Prx转录水平与细胞内ROS水平之间呈负相关。2-DE蛋白质组学分析的结果证实，500 µM H ₂ O ₂处理下调了2-Cys Prx的表达，并引起了对滨海假丝酵母光合能力的更大损害。蝇。