Prostaglandins (PG) have primary functions in the reproductive tract, however, the mechanism of regulation of PG secretion in the endometrium is unclear. Estrogen as a predominant regulator of uterine functions during the mammalian estrous cycle and effects of estrogen on synthesis of PG and function in uterine tissues of cattle are not fully understood. In this study, there was evaluation of the concentration- and time-effects of 17β-estradiol on PG synthesis in endometrial explants of cattle, focusing on the secretion of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) as well as relative abundance of mRNA transcript and protein for both the enzymes responsible for PGE2 and PGF2α synthesis, including prostaglandin-endoperoxide synthase 1 and 2 (PTGS1, PTGS2), PGE2 synthase (PGES), PGF2α synthase (PGFS), and carbonyl reductase (CBR1), and the receptors responsible for downstream PGE2 (PTGER2, PTGER4) and PGF2α (PTGFR) signaling. Results indicated that 17β-estradiol increased PGE2 and PGF2α production at concentrations ranging from 10-11 to 10-8 M. Furthermore, abundances of PTGS1, PTGS2, PGES, PGFS, PTGER2, PTGER4, and PTGFR mRNA transcripts and protein were greater immediately after 17β-estradiol treatment at almost all the concentrations, while these CBR1 abundances were less as a result of treatments with 17β-estradiol. These data support the hypothesis that estradiol modulates the synthesis and function of PG in the endometrium of cattle.

中文翻译：

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17β-雌二醇调节牛子宫内膜外植体中前列腺素E2和F2α的合成和功能。

前列腺素（PG）在生殖道中具有主要功能，但是，子宫内膜中PG分泌的调节机制尚不清楚。雌激素在哺乳动物动情周期中是子宫功能的主要调节剂，并且雌激素对牛的PG合成和子宫组织功能的影响尚不完全清楚。在这项研究中，评估了17β-雌二醇对牛子宫内膜外植体中PG合成的浓度和时间影响，重点是前列腺素E2（PGE2）和前列腺素F2α（PGF2α）的分泌以及其相对丰度。负责PGE2和PGF2α合成的酶的mRNA转录物和蛋白质，包括前列腺素-内过氧化物合酶1和2（PTGS1，PTGS2），PGE2合酶（PGES），PGF2α合酶（PGFS）和羰基还原酶（CBR1），以及负责下游PGE2（PTGER2，PTGER4）和PGF2α（PTGFR）信号的受体。结果表明，17β-雌二醇在10-11 M至10-8 M的浓度下增加PGE2和PGF2α的产生。此外，PTGS1，PTGS2，PGES，PGFS，PTGER2，PTGER4和PTGFR mRNA的丰度在紧随其后立即增加17β-雌二醇几乎在所有浓度下均可治疗，而这些CBR1丰度则因17β-雌二醇处理而减少。这些数据支持了雌二醇调节牛子宫内膜中PG的合成和功能的假说。在几乎所有浓度下，17β-雌二醇处理后，PTGS1，PTGS2，PGES，PGFS，PTGER2，PTGER4和PTGFR mRNA的丰度立即升高，而这些CBR1的丰度由于17β-雌二醇的处理而降低。这些数据支持了雌二醇调节牛子宫内膜中PG的合成和功能的假说。在几乎所有浓度下，17β-雌二醇处理后，PTGS1，PTGS2，PGES，PGFS，PTGER2，PTGER4和PTGFR mRNA的丰度立即升高，而这些CBR1的丰度由于17β-雌二醇的处理而降低。这些数据支持了雌二醇调节牛子宫内膜中PG的合成和功能的假说。