This study evaluated how Proteus vulgaris affects sperm quality and sperm-bacteria interaction in stored semen samples. A strain of P. vulgaris resistant to streptomycin, penicillin, lincomycin and spectinomycin was added to boar semen in doses of 103, 105, 106, 107 and 108 CFU/mL. A sample in which there was no addition of P. vulgaris was the negative control. Sperm quality was determined by evaluating sperm motility and morphology using the computer-assisted sperm analysis (CASA) system, and plasma membrane and acrosome integrity using flow cytometry at 0, 1, 2, 4, 6, 8 and 10 days of liquid-storage at 17 °C. At the same time points, pH, sperm agglutination, sperm-bacteria interaction and bacterial growth were also assessed. There was impaired sperm motility when the infective doses of P. vulgaris were equal to or greater than 105 CFU/mL with the effect being dose-dependent (P < 0.05). When infective doses of P. vulgaris were of 106 to 108 CFU/mL there were impairments of plasma membrane and acrosome integrity by Day 1 of storage (P < 0.05), and alkalinisation of the storing medium by Day 4 (P < 0.05). Bacterial adhesion increased when infective dose of P. vulgaris was greater and as duration of storage increased. P. vulgaris had a high affinity for the mid- and principal pieces of sperm cells. It is concluded there were alterations in sperm motility in samples infected with P. vulgaris that were associated with bacterial adhesion and medium alkalinisation.

中文翻译：

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长期储存受寻常变形杆菌污染的公猪精液：对精子活力和精子-细菌相互作用的剂量依赖性作用。

这项研究评估寻常变形杆菌如何影响精液样品中精子质量和精子与细菌的相互作用。以103、105、106、107和108 CFU / mL的剂量向猪精液中添加对链霉素，青霉素，林可霉素和大观霉素具有抗性的寻常疟原虫菌株。不添加寻常型毕赤酵母的样品为阴性对照。通过使用计算机辅助的精子分析（CASA）系统评估精子的活动性和形态来确定精子质量，并在储液的0、1、2、4、6、8和10天使用流式细胞术评估质膜和顶体完整性在17°C。同时，还评估了pH，精子凝集，精子-细菌相互作用和细菌生长。当感染剂量为P时，精子活力受损。寻常型等于或大于105 CFU / mL，且效果取决于剂量（P <0.05）。当寻常型假单胞菌的感染剂量为106至108 CFU / mL时，在储存的第1天时质膜和顶体完整性受到损害（P <0.05），在储存的第4天时碱化了储存介质（P <0.05）。当寻常型毕赤酵母的感染剂量更大时，随着储存时间的增加，细菌的粘附性也会增加。寻常疟原虫对精子的中段和主要部分具有很高的亲和力。结论是感染了寻常型毕赤酵母的样品中精子活力发生了变化，这与细菌粘附和中等碱化有关。寻常型为106至108 CFU / mL，在储存的第1天时质膜和顶体完整性受损（P <0.05），在储存的第4天时碱化了储存介质（P <0.05）。当寻常型毕赤酵母的感染剂量更大时，随着保存时间的增加，细菌的粘附性也会增加。寻常疟原虫对精子的中段和主要部分具有很高的亲和力。结论是感染了寻常型毕赤酵母的样品中精子活力发生了变化，这与细菌粘附和中等碱化有关。寻常型为106至108 CFU / mL，在储存的第1天时质膜和顶体完整性受到损害（P <0.05），在储存的第4天时碱化了储存介质（P <0.05）。当寻常型毕赤酵母的感染剂量更大且随着储存时间增加时，细菌粘附增加。寻常疟原虫对精子的中段和主要部分具有很高的亲和力。结论是感染了寻常型毕赤酵母的样品中精子活力发生了变化，这与细菌粘附和中等碱化有关。