The aim of this study was to evaluate effects of selection using the Percoll density gradient method on motility, mitochondrial membrane potential (ΔΨMit) and fertility in a subpopulation of testicular spermatozoa obtained from Atlantic salmon (Salmo salar). Samples were divided into three groups: Control (C), T1 (45/90 % Percoll®) and T2 (45/60 % Percoll®). Sperm motility was evaluated using CASA (Computer-Assisted Sperm Analysis), ΔΨMit using flow cytometry, and fertility evaluating whether cleavage of fertilised eggs had occurred after 16 h of incubation at 10 °C. Results indicate that motility was greater in T1 (92 ± 2.91 %) and T2 (89 ± 2.88 %) than in the Control (83.2 ± 2.04 %). The percentage of ΔΨMit was 88.3 ± 0.58 % and 85 ± 2% for T1 and T2, respectively, compared to 35 ± 6.24 % for the control. The fertility rates were 76 ± 9.1 % and 70 ± 8.1 % for T1 and T2, respectively, compared with 66 ± 12 % for the control. The kinetic characteristics for T1 were curvilinear velocity (VCL): 92.44 ± 21.12 μm/s, average path velocity (VAP): 85.87 ± 21.83 μm/s; and for T2 VCL was 78.69 ± 17.63 μm/s and VAP was 73.62 ± 17.08 μm/s. The results indicate sperm motility and ΔΨMit were greater in T1 and T2 compared with the control (P < 0.05). Similarly, there was an increase in the fertilisation rate compared to the control. The results from this study are the first where sperm quality variables were evaluated for Salmo salar testicular sperm using the Percoll® density gradient method.

中文翻译：

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Percoll密度梯度方法的选择对大西洋鲑（Salmo salar）睾丸精子亚群的活力，线粒体膜电位和生育力的影响。

这项研究的目的是评估使用Percoll密度梯度法进行选择对大西洋鲑（Salmo salar）睾丸精子亚群的活力，线粒体膜电位（ΔpotentialMit）和生育力的影响。样品分为三组：对照（C），T1（45/90％Percoll®）和T2（45/60％Percoll®）。使用CASA（计算机辅助精子分析）评估精子活力，使用流式细胞仪评估ΔΨMit，并在10°C孵育16小时后评估是否受精卵分裂。结果表明，与对照组相比，T1（92±2.91％）和T2（89±2.88％）的运动能力更大。T1和T2的ΔΨMit百分比分别为88.3±0.58％和85±2％，而对照组为35±6.24％。生育率为76±9。T1和T2分别为1％和70±8.1％，而对照组为66±12％。T1的动力学特性为曲线速度（VCL）：92.44±21.12μm/ s，平均路径速度（VAP）：85.87±21.83μm/ s; 对于T2，VCL为78.69±17.63μm/ s，VAP为73.62±17.08μm/ s。结果表明，与对照组相比，T1和T2的精子活力和ΔitMit更高（P <0.05）。类似地，与对照相比，受精率增加。该研究的结果是首次使用Percoll®密度梯度法评估Salmo ar睾丸精子的精子质量变量。87±21.83μm/ s; 对于T2，VCL为78.69±17.63μm/ s，VAP为73.62±17.08μm/ s。结果表明，与对照组相比，T1和T2的精子活力和ΔitMit更高（P <0.05）。类似地，与对照相比，受精率增加。该研究的结果是首次使用Percoll®密度梯度法评估Salmo ar睾丸精子的精子质量变量。87±21.83μm/ s; 对于T2，VCL为78.69±17.63μm/ s，VAP为73.62±17.08μm/ s。结果表明，与对照组相比，T1和T2的精子活力和ΔitMit更高（P <0.05）。类似地，与对照相比，受精率增加。这项研究的结果是首次使用Percoll®密度梯度法评估Salmo ar鳍睾丸精子的精子质量变量。