The aim of this study was to investigate relative abundance and localization of ISG15 mRNA transcript in intra-uterine (trophoblast, endometrium) and extra-uterine (hypothalamus, anterior pituitary, corpus luteum) tissues before and during the period of conceptus implantation. Multiparous ewes (n = 16) were randomly allotted into four groups: pregnant or estrous cyclic on days of 12 and 16 (n = 4 per group) following estrus. Relative abundances of ISG15 mRNA transcript were determined in the endometrium, corpus luteum, hypothalamus, and anterior-pituitary using real time quantitative PCR. Localization of ISG15 mRNA transcript was evaluated using in situ hybridization. The presence of ISG15 mRNA transcript was only visualized in intra-uterine tissues including the endometrium and trophoblast on day 12 of pregnancy. The ISG15 mRNA transcript was detected in all tissues evaluated on day 16 of pregnancy. The abundance of ISG15 mRNA transcript was greater in the endometrium on day 12 of pregnancy than at other days when evaluations occurred while in all other tissues except the hypothalamus there were large abundances of ISG15 mRNA on day 16 of pregnancy. It is concluded that the ISG15 mRNA transcript is only present in intra-uterine tissues before conceptus implantation. The ISG15 mRNA transcript, however, is present in extra-uterine tissues of ewes during implantation probably due to an increased amount of interferon-tau in blood circulation that is produced by the developing embryo. Results also indicate, for the first time, that pregnancy is associated with an intra-hypothalamus and anterior pituitary increased abundance of ISG15 mRNA transcript in ewes.

中文翻译：

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绵羊妊娠早期子宫内和子宫外组织中干扰素刺激的基因15 mRNA转录物的相对丰度和定位。

这项研究的目的是调查在子宫植入之前和期间，子宫内（滋养层，子宫内膜）和子宫外（下丘脑，垂体前叶，黄体）组织中ISG15 mRNA转录物的相对丰度和定位。将多头母羊（n = 16）随机分为四组：发情后第12天和第16天有妊娠或发情周期（每组n = 4）。使用实时定量PCR测定子宫内膜，黄体，下丘脑和垂体前叶中ISG15 mRNA转录物的相对丰度。使用原位杂交评估ISG15 mRNA转录物的定位。ISG15 mRNA转录物的存在仅在妊娠第12天时在子宫内组织（包括子宫内膜和滋养细胞）中可见。在妊娠第16天评估的所有组织中均检测到ISG15 mRNA转录物。妊娠第12天子宫内膜的ISG15 mRNA转录物丰度要高于其他天数，而除下丘脑以外的所有其他组织在妊娠第16天都有大量ISG15 mRNA的丰度。结论是，ISG15 mRNA转录物仅在概念植入前的子宫内组织中存在。但是，ISG15 mRNA转录物在植入过程中存在于母羊的子宫外组织中，这可能是由于发育中的胚胎在血液循环中产生的干扰素-tau数量增加所致。结果还首次表明，妊娠与母羊下丘脑内和垂体前叶ISG15 mRNA转录物丰度增加有关。