ABSTRACT

Aims: The analysis of the online databases revealed that CCND1 expression is correlated with poor prognosis in LSCC. We aimed to explore the function of CCND1 in tumor progression in LSCC.

Main methods: The expression of mRNA was measured using qRT-PCR. Protein expression was assessed by Western blot. Cell migration and invasion were assessed by transwell assay.

Key findings: CCND1 was co-overexpressed with FGFR1 in lung cancer patients. Overexpression of CCND1 promoted LSCC cell proliferation and metastasis. FGFR1 promoted the processes of EMT through AKT/MAPK signaling by targeting CCND1 in FGFR1-amplification cell lines.

Significance: IIn conclusion, our study demonstrated the regulatory mechanism between CCND1 and FGFR1 in FGFR1 amplified LSCC. Co-targeting CCND1 and FGFR1 could provide greater clinical benefits.

摘要

目的：对在线数据库的分析表明，CCND1表达与LSCC的不良预后相关。我们旨在探讨CCND1在LSCC肿瘤进展中的功能。

主要方法：用qRT-PCR检测mRNA的表达。通过蛋白质印迹评估蛋白质表达。通过transwell测定法评估细胞迁移和侵袭。

关键发现：在肺癌患者中CCND1与FGFR1共过表达。CCND1的过表达促进LSCC细胞增殖和转移。通过靶向FGFR1扩增细胞系中的CCND1，FGFR1通过AKT / MAPK信号传导促进了EMT过程。

意义：总之，我们的研究证明了FGFR1扩增的LSCC中CCND1和FGFR1之间的调控机制。共同靶向CCND1和FGFR1可提供更大的临床益处。