Expression of immune function genes within follicle cells has been reported in ovaries from many species. Recent work from our laboratory showed a direct effect of the monocyte chemoattractant protein 1/C-C motif chemokine receptor 2 system within the feline cumulus oocyte complex, by increasing the mRNA levels of key genes involved in the ovulatory cascade in vitro. Studies were designed to evaluate if C-C motif chemokine receptor 2 acts as a novel mediator of the ovulatory cascade in vitro. Therefore, feline cumulus oocyte complexes were cultured in the presence or absence of a highly selective C-C motif chemokine receptor 2 antagonist together with known inducers of cumulus-oocyte expansion and/or oocyte maturation to assess mRNA expression of key genes related to periovulatory events in other species as well as oocyte maturation. Also, the effects of recombinant monocyte chemoattractant protein 1 on spontaneous or gonadotrophin-induced oocyte maturation were assessed. This is an in vitro system using isolated cumulus oocyte complexes from feline ovaries. The present study reveals the modulation of several key ovulatory genes by a highly selective C-C motif chemokine receptor 2 antagonist. However, this antagonist was not enough to block the oocyte maturation induced by gonadotropins or amphiregulin. Nonetheless, recombinant monocyte chemoattractant protein 1 had a significant effect on spontaneous oocyte maturation, increasing the percentage of metaphase II stage oocytes in comparison to the control. This is the first study in any species to establish C-C motif chemokine receptor 2 as a mediator of some actions of the mid-cycle gonadotrophin surge.

中文翻译：

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CC 基序趋化因子受体 2 作为排卵级联中的新型中间体。

已经报道了许多物种的卵巢中卵泡细胞内免疫功能基因的表达。我们实验室最近的工作表明，通过增加体外排卵级联中关键基因的 mRNA 水平，单核细胞趋化蛋白 1/CC 基序趋化因子受体 2 系统在猫卵丘卵母细胞复合物中的直接作用。研究旨在评估 CC 基序趋化因子受体 2 是否作为体外排卵级联的新介质。因此，在存在或不存在高选择性 CC 基序趋化因子受体 2 拮抗剂以及已知的卵丘-卵母细胞扩张和/或卵母细胞成熟诱导剂的情况下培养猫卵丘卵母细胞复合物，以评估与其他排卵期事件相关的关键基因的 mRNA 表达。物种以及卵母细胞的成熟。还，评估了重组单核细胞趋化蛋白 1 对自发性或促性腺激素诱导的卵母细胞成熟的影响。这是一个体外系统，使用来自猫卵巢的分离的卵丘卵母细胞复合物。本研究揭示了高选择性 CC 基序趋化因子受体 2 拮抗剂对几个关键排卵基因的调节。然而，这种拮抗剂不足以阻断由促性腺激素或双调蛋白诱导的卵母细胞成熟。尽管如此，重组单核细胞趋化蛋白 1 对自发卵母细胞成熟有显着影响，与对照相比，增加了中期 II 期卵母细胞的百分比。这是在任何物种中建立 CC 基序趋化因子受体 2 作为中期促性腺激素激增某些作用的介质的第一项研究。