The spread of carbapenemase-producing Enterobacterales is a major public health concern worldwide, and methods for their prompt and reliable detection are highly demanded for therapeutic and hygiene control purposes. In this study, we evaluate the MBT STAR^®-Carba assay (Bruker Daltonik) to detect the carbapenemase production in clinical and surveillance isolates from plate cultures and directly from patient-derived positive blood cultures bottles. Overall, n = 1,307 samples were analyzed (n = 900 plate cultures, and n = 407 positive blood cultures, using the bacterial pellet obtained with the Sepsityper^® Kit; Bruker Daltonik), including n = 793 carbapenemase producers (n = 579 Klebsiella pneumoniae carbapenemase, n = 161 metallo-beta-lactamases, n = 45 OXA-48, and eight isolates harboring two different enzymes), n = 239 carbapenem-resistant noncarbapenemase producers, and n = 275 carbapenem-susceptible strains. The STAR-Carba assay detected 657/661 (99.4%) carbapenemase producers from plate cultures, and 132/132 (100%) from positive blood cultures. Specificity resulted in 100% for carbapenem-susceptible strains, and 91.6% for carbapenem-resistant strains resulted negative for carbapenamase production with the routine methods used in this study. In this study, the MBT STAR-Carba assay proved to be a highly reliable method for the detection of carbapenemase-producing Enterobacterales, regardless of the enzyme family, and from both plate cultures and positive blood culture bottles.

中文翻译：

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MBT STAR-Carba 测定法用于检测肠杆菌科和 Hafniaceae 中碳青霉烯酶的产生，并从平板培养物和患者来源的阳性血液培养物中收集大量常规分离物，对其进行评估。

产碳青霉烯酶肠杆菌的传播是世界范围内一个主要的公共卫生问题，为了治疗和卫生控制目的，迫切需要快速可靠地检测它们的方法。在本研究中，我们评估了 MBT STAR ^® -Carba 测定 (Bruker Daltonik)，以检测来自平板培养物和直接来自患者阳性血培养瓶的临床和监测分离株中碳青霉烯酶的产生。总体而言， 分析了n = 1,307 个样本（n  = 900 个平板培养物，n  = 407 个阳性血培养物，使用通过 Sepsityper ^®试剂盒获得的细菌沉淀；Bruker Daltonik），包括n = 793 种碳青霉烯酶生产者（n  = 579肺炎克雷伯菌碳青霉烯酶，n  = 161 种金属-β-内酰胺酶，n  = 45 OXA-48，以及 8 个含有两种不同酶的分离株），n  = 239 种耐碳青霉烯非碳青霉烯酶生产者，以及n = 275 种碳青霉烯敏感菌株。STAR-Carba 检测从平板培养中检测到 657/661 (99.4%) 碳青霉烯酶生产者，从阳性血培养中检测到 132/132 (100%)。使用本研究中使用的常规方法，碳青霉烯敏感菌株的特异性为 100%，碳青霉烯抗性菌株的特异性为 91.6%，导致碳青霉烯酶产生阴性。在本研究中，MBT STAR-Carba 检测被证明是一种高度可靠的方法，可用于检测产碳青霉烯酶的肠杆菌，无论酶家族如何，无论是平板培养还是阳性血培养瓶。