Methods previously developed by the author are applied to uncover several sites of interest in the spike glycoproteins of all known human coronaviruses (hCoVs), including SARS-CoV-2 that causes COVID-19. The sites comprise three-dimensional neighborhoods of peptides characterized by four key properties: (1) they pinpoint regions of high free energy in the backbone whose obstruction might interrupt function; (2) by their very definition, they occur rarely in the universe of all gene-encoded proteins that could obviate host response to compounds designed for their interference; (3) they are common to all known hCoV spikes, possibly retaining activity in light of inevitable viral mutation; and (4) they are exposed in the molecular surface of the glycoprotein. These peptides in SARS-CoV-2 are given by the triples of residues (131, 117, 134), (203, 227, 228), and (1058, 730, 731) in its spike.

中文翻译：

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人冠状病毒突状糖蛋白骨架中保守的高自由能位点。

作者先前开发的方法用于发现所有已知人类冠状病毒（hCoV）的突波糖蛋白中的几个目标位点，包括引起COVID-19的SARS-CoV-2。这些位点包括具有三个关键特性的肽的三维邻域：（1）它们确定骨架中高自由能的区域，该区域的阻塞可能会中断功能；（2）就其定义而言，它们很少出现在所有基因编码的蛋白质的宇宙中，这些蛋白质可以消除宿主对为其干扰而设计的化合物的反应；（3）它们是所有已知的hCoV峰值共有的，鉴于不可避免的病毒突变，它们可能保持活性；（4）它们暴露于糖蛋白的分子表面。SARS-CoV-2中的这些肽由残基（131、117、134），（203、227，