SIGNIFICANCE Recent generation of bioengineered human skin allowed the efficient treatment of patients with severe skin defects. However, the optical and biomechanical properties of these models are not known. AIM Three models of bioengineered human skin based on fibrin-agarose biomaterials (acellular, dermal skin substitutes, and complete dermoepidermal skin substitutes) were generated and analyzed. APPROACH Optical and biomechanical properties of these artificial human skin substitutes were investigated using the inverse adding-doubling method and tensile tests, respectively. RESULTS The analysis of the optical properties revealed that the model that most resembled the optical behavior of the native human skin in terms of absorption and scattering properties was the dermoepidermal human skin substitutes after 7 to 14 days in culture. The time-course evaluation of the biomechanical parameters showed that the dermoepidermal substitutes displayed significant higher values than acellular and dermal skin substitutes for all parameters analyzed and did not differ from the control skin for traction deformation, stress, and strain at fracture break. CONCLUSIONS We demonstrate the crucial role of the cells from a physical point of view, confirming that a bioengineered dermoepidermal human skin substitute based on fibrin-agarose biomaterials is able to fulfill the minimal requirements for skin transplants for future clinical use at early stages of in vitro development.

中文翻译：

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评估用纤维蛋白-琼脂糖生物材料生成的生物工程人体皮肤的光学和生物力学特性。


意义 最新一代的生物工程人体皮肤可以有效治疗患有严重皮肤缺陷的患者。然而，这些模型的光学和生物力学特性尚不清楚。目的 生成并分析了三种基于纤维蛋白-琼脂糖生物材料的生物工程人体皮肤模型（无细胞、真皮皮肤替代品和完全真皮表皮替代品）。方法分别使用逆加倍法和拉伸试验研究了这些人造皮肤替代品的光学和生物力学特性。结果 光学特性分析表明，在吸收和散射特性方面最类似于天然人类皮肤光学行为的模型是培养 7 至 14 天后的真皮表皮人类皮肤替代品。生物力学参数的时程评估表明，真皮表皮替代品在所有分析参数上都显示出明显高于脱细胞和真皮皮肤替代品的值，并且在牵引变形、应力和断裂应变方面与对照皮肤没有差异。结论我们从物理角度证明了细胞的关键作用，证实基于纤维蛋白-琼脂糖生物材料的生物工程真皮表皮人类皮肤替代品能够满足未来体外早期临床使用的皮肤移植的最低要求发展。