Abstract

The association of probiotics and fruit extracts may influence the chemopreventive effect of colorectal cancer. In this context, antiproliferative activity was evaluated to select the best extracts that would be added probiotics, after addition of Bifidobacterium or Lactobacillus in the extracts the antiradical and antioxidant activity, quinone reductase (QR) assay and apoptosis assay were evaluated. Four extracts were isolated: E1: rich in total phenolic compounds; E2: rich in water-soluble phenolic compounds; E3: rich in most apolar phenolic compounds and E4: rich in anthocyanins. The antiproliferative results showed that the best extracts for blueberry and jabuticaba were, respectively the extract E4 and E2. After addition of the probiotic bacteria in these best extracts, it was observed that E2 from jabuticaba presented significantly higher antiradical and antioxidant activity values compared to E4 from blueberry before and after addition of probiotics. There was also a 9-fold increase in activity of QR by the E2 from jabuticaba with Lactobacillus (JL). Likewise, this same extract showed a significant increase both in apoptotic and necrotic cells for both cells. In conclusion, extract rich in water-soluble phenolic compounds (E2) from jabuticaba presented a greater chemopreventive effect compared to the others.

摘要

益生菌和水果提取物的结合可能会影响结直肠癌的化学预防效果。在这种情况下，在添加双歧杆菌或乳杆菌后，评估了抗增殖活性以选择最好的提取物来添加益生菌在提取物中的抗自由基和抗氧化活性，醌还原酶 (QR) 测定和细胞凋亡测定进行了评估。分离出四种提取物： E1：富含总酚类化合物；E2：富含水溶性酚类化合物；E3：富含大多数非极性酚类化合物和 E4：富含花青素。抗增殖结果表明，蓝莓和jabuticaba的最佳提取物分别是提取物E4和E2。在这些最佳提取物中添加益生菌后，观察到与添加益生菌前后的蓝莓 E4 相比，来自 jabuticaba 的 E2 表现出显着更高的抗自由基和抗氧化活性值。来自 jabuticaba 的 E2 与乳杆菌的 QR 活性也增加了 9 倍(JL)。同样，同一提取物显示两种细胞的凋亡和坏死细胞均显着增加。总之，与其他提取物相比，富含水溶性酚类化合物 (E2) 的 jabuticaba 提取物具有更大的化学预防作用。