We investigated the antibiotic-resistance phenotypes and molecularly characterized class 1 integron gene cassettes from 113 Pseudomonas aeruginosa isolates from patients. Primers specific for the class 1 integron integrase (intI1) gene were used to screen for these integrons using polymerase chain reactions (PCRs). The variable regions of the integrons were PCR-amplified and sequenced. Sputum was the most common specimen (69.9%; 79/113) followed by aseptic sites (21.2%; 24/113). Of the 113 isolates with phenotypic resistance to the tested antimicrobials, the highest resistances were to ciprofloxacin (CIP) (26.55%), imipenem (IPM) (23.89%), and meropenem (MEM) (23%). Carbapenem-sensitive P. aeruginosa (CS-PA) isolates displayed 23 patterns, and the predominant multidrug resistance phenotype was CIP-levofloxacin (7.23%, 6/83). Carbapenem-resistant P. aeruginosa (CR-PA) isolates displayed 12 patterns, and the predominant multidrug resistance phenotype was IPM-MEM (23.33%, 7/30). Class 1 integrons were detected in 14 (12.4%, 14/113) isolates, 7.22% (6/83) in CS-PA isolates, and 26.67% (8/30) in CR-PA isolates. Six gene cassette arrays were detected, the most prevalent being aacA4-blaOXA101-aadA5 in five isolates (4.4%, 5/113). Seventeen gene cassettes were detected. The most prevalent antibiotic-resistance gene cassettes were aacA4 (6.2%, 7/113), bla_OXA-1, and bla_OXA-101. Extended-spectrum β-lactamase resistance genes were detected. Some of the genes carried were similar to those in other species, but some had shared characteristics among the P. aeruginosa isolates. Long-standing drug resistance genes appeared to be under elimination in P. aeruginosa, whereas integrons conferring resistance to commonly used clinical drugs such as β-lactamases, fluoroquinolones, and even carbapenems, as well as some other gene elements, were found to be newly integrated.

中文翻译：

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铜绿假单胞菌菌株1类整合子的基因盒的抗药性和分子表征。

我们调查了来自患者的113株铜绿假单胞菌分离株的抗生素抗性表型和分子表征的1类整合子基因盒。使用聚合酶链反应（PCR），使用第1类整合子整合酶（intI1）基因特异的引物筛选这些整合子。对整合素的可变区进行PCR扩增和测序。痰是最常见的标本（69.9％； 79/113），其次是无菌部位（21.2％； 24/113）。在对测试抗生素具有表型耐药性的113个分离物中，对环丙沙星（CIP）（26.55％），亚胺培南（IPM）（23.89％）和美洛培南（MEM）（23％）的耐药性最高。碳青霉烯敏感铜绿假单胞菌（CS-PA）分离株表现出23种模式，主要的多药耐药表型为CIP-左氧氟沙星（7.23％，6/83）。耐碳青霉烯的铜绿假单胞菌（CR-PA）分离株显示12种模式，主要的多药耐药表型为IPM-MEM（23.33％，7/30）。在14个（12.4％，14/113）分离株，CS-PA分离株中7.22％（6/83）和CR-PA分离物中26.67％（8/30）中检测到1类整合素。检测到六个基因盒阵列，其中最常见的是五个分离株中的aacA4-blaOXA101-aadA5（4.4％，5/113）。检测到十七个基因盒。最普遍的抗生素耐药基因盒是aacA4（6.2％，7/113），bla _OXA-1和bla _OXA-101。检测到广谱β-内酰胺酶抗性基因。携带的一些基因与其他物种相似，但一些在铜绿假单胞菌分离物中具有共同的特征。长期存在的耐药基因似乎在铜绿假单胞菌中被消除，而赋予对常用临床药物如β-内酰胺酶，氟喹诺酮，甚至碳青霉烯类药物以及其他一些基因元件的耐药性的整合素是新发现的。集成。