The work reported in this research communication investigated the occurrence of Mycoplasma bovis (M. bovis) in milk samples from cows with clinical mastitis on dairy farms from seven Brazilian states. We hypothesized that M. bovis was present in bovine clinical mastitis milk in Brazil. A total of 561 milk samples were cultured on Hayflick agar and incubated in a microaerophilic atmosphere at 5% CO2. Polymerase chain reaction (PCR) was performed for the detection of Mycoplasma spp. and Mycoplasma bovis in milk samples. Mycoplasma spp. were isolated in 2% of the milk samples, and Mycoplasma bovis was verified in 3% of the milk samples by PCR. The results showed that Mycoplasma bovis is involved in clinical mastitis in Brazilian dairy herds. We emphasize the need for further studies to investigate the infection by this agent in clinical mastitis cases, particularly in Brazil, due to the lack of knowledge about its prevalence.

中文翻译：

---

巴西牛支原体引起的临床牛乳腺炎检测

本研究通讯中报告的工作调查了牛支原体(牛分枝杆菌) 在来自巴西七个州的奶牛场的临床乳腺炎奶牛的牛奶样本中。我们假设牛分枝杆菌存在于巴西的牛临床乳腺炎牛奶中。总共有 561 个牛奶样品在 Hayflick 琼脂上培养，并在 5% CO 的微需氧气氛中培养2. 聚合酶链式反应 (PCR) 用于检测支原体spp. 和牛支原体在牛奶样品中。支原体spp. 在 2% 的牛奶样品中被分离出来，并且牛支原体通过 PCR 在 3% 的牛奶样品中进行了验证。结果表明，牛支原体参与了巴西奶牛群的临床乳腺炎。我们强调需要进一步研究以调查这种药物在临床乳腺炎病例中的感染情况，特别是在巴西，因为对其流行程度缺乏了解。