A fluorescent probe to detect biothiols (GSH/Hcy/Cys) was synthesized with benzothiazole as the fluorophore and nitromethane as the recognition group. The recognition mechanism of the fluorescent probe was deduced. It was found that a nitroalkene in the molecule was used as the recognition site for this probe and reacted with the mercapto group of the biothiols by electrophilic addition, significantly enhancing the fluorescent signal. Experimental results showed that the fluorescent probe had a low detection limit, good selectivity, strong anti‐interference ability, and good naked eye recognition. The probe could detect biothiols (GSH/Hcy/Cys) in 20% organic solvent, with detection limits of 0.33 μM, 0.70 μM, and 0.87 μM, respectively. The probe will be applied further in biosensors and other fields.

中文翻译：

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用于肉眼识别的基于苯并恶唑衍生物的荧光探针的合成和应用。

以苯并噻唑为荧光团，以硝基甲烷为识别基团，合成了一种检测生物硫醇的荧光探针（GSH / Hcy / Cys）。推导了荧光探针的识别机理。发现分子中的硝基烯烃被用作该探针的识别位点，并通过亲电加成与生物硫醇的巯基反应，显着增强了荧光信号。实验结果表明，该荧光探针检测限低，选择性好，抗干扰能力强，肉眼识别能力强。该探针可以检测20％有机溶剂中的生物硫醇（GSH / Hcy / Cys），检测极限分别为0.33μM，0.70μM和0.87μM。该探针将进一步应用于生物传感器和其他领域。