The wide use of cobalt (Co) in food, industry, and medical devices requires full elucidation of its biological effects on tissues and organs. The aim was to assess serum metabolic alterations in immature mice after subchronic exposure to CoCl₂. Pregnant ICR mice were subjected to a daily dose of 75 mg cobalt chloride/kg body weight (CoCl₂x6H₂O) 2–3 days before they gave birth, and treatment continued until days 25 and 30 after delivery. The compound was dissolved in and obtained with regular tap water. ICP-DRC-MS analysis showed significantly elevated serum Co²⁺ and diverse alterations in metabolic parameters of 25- and 30-day-old pups after exposure to CoCl₂. Cholesterol and urea levels were significantly elevated in day 25 mice while HDL-C and LDL-C were reduced. In day 30, Co-exposed mice LDL-C and triglycerides were significantly increased while the total cholesterol level remained unchanged. Alkaline phosphatase was significantly reduced in day 25 Co-exposed mice. Blood glucose level of Co-exposed mice remained close to the untreated controls. Total protein content was slightly increased in day 30 mice. Co-exposure reduced albumin content and albumin/globulin ratio but increased significantly globulin content. Co administration showed strong correlation with cholesterol, urea, and HDL-C in both day 25 and 30 mice. Inverse correlation was found with alkaline phosphatase and albumin for day 25 and with triglycerides, globulin, and total protein content in day 30 Co-exposed mice. Subchronic CoCl₂ exposure of immature mice induced significant changes in key metabolic parameters suggesting possible further disturbances in energy metabolism, osteogenesis, and reproduction.

钴（Co）在食品，工业和医疗设备中的广泛使用要求充分阐明其对组织和器官的生物学作用。目的是评估亚慢性暴露于CoCl ₂后未成熟小鼠的血清代谢变化。怀孕的ICR小鼠在分娩前₂至3天每天接受75 mg氯化钴/ kg体重（CoCl ₂ x6H ₂ O）的日剂量，治疗持续至分娩后25天和30天。将化合物溶解并用常规自来水获得。ICP-DRC-MS分析显示，暴露于CoCl ₂后25和30日龄幼犬的血清Co ²⁺明显升高，代谢参数发生多种变化。第25天小鼠的胆固醇和尿素水平显着升高，而HDL-C和LDL-C降低。在第30天，共同暴露的小鼠LDL-C和甘油三酸酯显着增加，而总胆固醇水平保持不变。在第25天同时暴露的小鼠中，碱性磷酸酶显着降低。共同暴露的小鼠的血糖水平保持接近未处理的对照。在第30天的小鼠中，总蛋白含量略有增加。共同暴露降低白蛋白含量和白蛋白/球蛋白比，但显着增加球蛋白含量。在第25天和30天的小鼠中，共同给药显示与胆固醇，尿素和HDL-C密切相关。第25天与碱性磷酸酶和白蛋白呈负相关，在第30天共暴露的小鼠中与甘油三酸酯，球蛋白和总蛋白含量呈负相关。亚慢性CoCl_2次未成熟小鼠的暴露引起关键代谢参数的显着变化，表明能量代谢，成骨和繁殖可能进一步受到干扰。