Sedentary behavior increases the risk of many chronic disorders, in addition, these chronic diseases are associated with elevated markers interleukin-6 (IL-6). Increasing evidence indicates that physical activity can prevent chronic inflammatory disease. However, the effect of exercise on sedentary individuals with disparate basal serum IL-6 level was not well elucidated. In this study, the gene expression profile of GES12384 was downloaded from the Gene Expression Omnibus (GEO) database. This data set contained 12 sedentary middle-aged men (6 high IL-6 and 6 low IL-6 level), and their blood samples were taken in the pre-exercise period and at the end of 24 weeks of exercise. The differentially expressed genes (DEGs) of 24 weeks group were identified, followed by functional enrichment analysis. Subsequently, protein–protein interaction (PPI) network and transcription factors (TFs)-DEGs network were constructed. A total of 193 DEGs were identified between high and low IL-6 level in the 24 weeks group. Functional enrichment analysis showed that DEGs were mainly involved in African trypanosomiasis pathway. PPI network revealed that the hub genes included C-C motif chemokine receptor 7 (CCR7), hemoglobin subunit delta (HBD), and interferon gamma (IFNG). Subnetworks analysis indicated that these genes were relevant to immune response, and participated in African trypanosomiasis pathway. The TF targets network found that myocyte enhancer factor 2A (MEF2A) was a key regulatory factor. In conclusion, the inflammation-related genes (CCR7, HBD, and IFNG) in sedentary individuals could be affected by exercise, and the identified DEGs and TFs in this study promoted our understanding of exercise inhibited the development of chronic disease.

中文翻译：

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久坐不动的人运动引起的白细胞介素6水平相关基因和途径的变化。

久坐的行为会增加许多慢性疾病的风险，此外，这些慢性疾病还与白细胞介素6（IL-6）升高有关。越来越多的证据表明，体育锻炼可以预防慢性炎症性疾病。然而，运动对久坐的人具有不同的基础血清IL-6水平的影响尚不清楚。在这项研究中，GES12384的基因表达谱是从“基因表达综合”（GEO）数据库下载的。该数据集包含12位久坐的中年男性（6位高IL-6和6位低IL-6水平），他们的血液样本是在运动前和运动24周结束时采集的。鉴定24周组的差异表达基因（DEG），然后进行功能富集分析。后来，构建了蛋白质-蛋白质相互作用（PPI）网络和转录因子（TFs）-DEGs网络。在24周组中，在高和低IL-6水平之间共鉴定出193个DEG。功能富集分析表明，DEGs主要参与非洲锥虫病传播途径。PPI网络显示，集线器基因包括CC基序趋化因子受体7（CCR7），血红蛋白亚基δ（HBD）和干扰素γ（IFNG）。子网分析表明，这些基因与免疫应答有关，并参与了非洲锥虫病传播途径。TF靶标网络发现心肌细胞增强因子2A（MEF2A）是关键的调节因子。总之，久坐者的炎症相关基因（CCR7，HBD和IFNG）可能会受到运动的影响，并且在这项研究中鉴定出的DEGs和TFs促进了我们对运动的认识抑制了慢性病的发展。