Chikungunya virus (CHIKV) is a mosquito‐borne virus that causes arthralgic fever. Fibroblast‐like synoviocytes play a key role in joint damage in inflammatory arthritides and can additionally serve as target cells for CHIKV infection. To gain a better understanding of CHIKV‐induced arthralgia, the interaction between CHIKV and synoviocytes was investigated at the protein level. A gel‐enhanced liquid chromatography‐mass spectrometry (GeLC‐MS/MS) approach was used to examine protein expression from primary human fibroblast‐like synoviocytes (HFLS) infected with clinical isolates of CHIKV at 12 and 24 hr post infection. Our analysis identified 259 and 241 proteins of known function that were differentially expressed (>1.5 or <−1.5 fold change) following CHIKV infection at 12 and 24 hpi, respectively. These proteins are involved in cellular homeostasis, including cellular trafficking, cytoskeletal organization, immune response, metabolic process, and protein modification. Some of these proteins have previously been reported to participate in arthralgia/arthritis and the death of infected cells. Our results provide information on the CHIKV‐induced modulation of cellular proteins of HFLS at an early stage of infection, as well as highlighting biological processes associated with CHIKV infection in the main target cells of the joint.

中文翻译：

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感染CHIKV的人类成纤维样滑膜细胞的蛋白质组学分析：鉴定可能与CHIKV复制和细胞发病机制相关的宿主因子。

基孔肯雅热病毒（CHIKV）是一种蚊媒病毒，可引起关节热。成纤维细胞样滑膜细胞在炎性关节炎的关节损伤中起关键作用，并且还可以作为CHIKV感染的靶细胞。为了更好地了解CHIKV引起的关节痛，我们在蛋白质水平上研究了CHIKV与滑膜细胞之间的相互作用。凝胶增强液相色谱-质谱（GeLC-MS / MS）方法用于检查感染后12和24小时感染CHIKV临床分离株的原代人成纤维样滑膜细胞（HFLS）的蛋白表达。我们的分析确定了259和241个已知功能的蛋白质，分别在12和24 hpi感染CHIKV后差异表达（> 1.5或<-1.5倍变化）。这些蛋白质参与细胞稳态，包括细胞运输，细胞骨架组织，免疫应答，代谢过程和蛋白质修饰。这些蛋白质中的一些以前已经被报道参与关节痛/关节炎和被感染细胞的死亡。我们的结果提供了有关CHIKV感染早期阶段HFLS细胞蛋白的细胞蛋白调制的信息，以及在关节主要靶细胞中突出显示了与CHIKV感染相关的生物学过程。